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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,628 results for: nin

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Showing: 301 - 320 of 66628 results

  2. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11518-plasmid-map-sequence-id-181065

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  3. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Publication
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100547-plasmid-map-sequence-id-313417

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  7. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103871-plasmid-map-sequence-id-259670

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  12. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52970-plasmid-map-sequence-id-82408

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Showing: 301 - 320 of 66628 results