58,076 results
-
-
-
pcDNA3.1-CD81-(TEV)-FKBP-SmBiT
Plasmid#182651Purposehuman CD81-TEV cleavage site-FKBP-SmBiT tagDepositorInserthuman CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Synthetic, Human)
UseTagsSmBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-wtSrc
Plasmid#140295PurposeExpresses Flag-tagged wild-type human Src in mammalian cells for structure-function studiesDepositorInsertSrc wildtype (SRC Human)
UseTags2X FlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-YFP
Plasmid#18826DepositorInsertTRPML1 (MCOLN1 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralTagsExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-LY-P2A
Plasmid#214895PurposeExpresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sitesDepositorInsertSPLICSFAST-LDh-LY-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iM
Plasmid#58972PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoter and IRESAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-HIF1B (#930)
Plasmid#99916Purposemammalian expressionDepositorInsertHIF1B (ARNT Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE mCherry-Rab11A Q70L
Plasmid#228038PurposeMammalian lentiviral expression of mCherry-labeled Rab11 Q70L (constitutively active mutation).DepositorInsertRab11A (RAB11A Human)
UseLentiviralTagsExpressionMammalianMutationQ70LPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Tmem192-mRFP-3xHA
Plasmid#134631Purposebait for affinity-isolation of lysosomesDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3xHA, TEV, and mRFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FKBP-FKBP-CFP (LF2C)
Plasmid#20149DepositorInsertLyn-FKBP-FKBP-CFP (FKBP1A Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA
Plasmid#89460Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorInsertCHD7 (CHD7 Human)
UseTagshinge + HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRbeta
Plasmid#136455PurposeMammalian expression plasmid for c-myc-tagged PDGFRBDepositorInsertPDGFRbeta (PDGFRB Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3495
Plasmid#144971PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCLOCK (CLOCK Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2745
Plasmid#143605PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHELZ2 (PRIC285 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2
Plasmid#167296PurposePlasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFPDepositorInsertBAX (BAX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXD3
Plasmid#153118PurposeExpression of Flag tagged human FOXD3 in mammalian cellsDepositorInserthuman FOXD3 (FOXD3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaV1.2/pcDNA3.1~ Human
Plasmid#140576PurposeExpression in mammallian cells or oocytesDepositorInserthuman CaV1.2 (CACNA1C Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only
