We narrowed to 18,548 results for: MUT

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pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Rev

Plasmid

#112811

Purpose

gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Keratin 8 G61C

Plasmid

#18055

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

G61C

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pSG 413

Plasmid

#63859

Purpose

Detect translation initiation from CUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

CUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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3087 pBS Bcl-2-Ig

Plasmid

#8809

Depositor

Stanley Korsmeyer

Article

Seto et al EMBO J 1988 Jan;7(1):123-31.

Insert

Bcl-2-Ig (BCL2 Human)

Expression

Bacterial

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
YFP-DAT R60A

Plasmid

#90247

Purpose

Expresses YFP-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

mutated R60 to A in human DAT cDNA

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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Keratin 8 S23A

Plasmid

#18057

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Mutation

S23A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pTTR210K

Plasmid

#41750

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; R210K

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8-DJ1-M26I

Plasmid

#29357

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Entry vector

Mutation

Met26Ile

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-E64D

Plasmid

#29352

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Glu64Asp

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hFX-promotor(1kb)

Plasmid

#14978

Depositor

Michael Ristow

Insert

human frataxin promoter fragment (FXN Human)

Use

Luciferase

Mutation

start codon for human frataxin gene has been muta…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pSG 417

Plasmid

#63864

Purpose

Detect translation initiation from AUU start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUU start codon

Promoter

PtacI

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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p-1459-adcy4-Luc

Plasmid

#28184

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

Adcy4 promoter (Adcy4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pSG 416

Plasmid

#63863

Purpose

Detect translation initiation from AUA start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUA start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pTGC-U6.2

Plasmid

#112809

Purpose

PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:2-gRNAcore

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-p65T305A,S276A

Plasmid

#106456

Purpose

NF-kB p65, kinase sites 276,305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase sites 276,305 mutated (RELA Human)

Expression

Mammalian

Mutation

kinase sites inactivated

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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CP1300-CBEmax

Plasmid

#119805

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1300) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSL14 - Prp8 2142–2398

Plasmid

#90264

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

PRP8 (PRP8 Budding Yeast)

Tags

N-terminal 6xHis tag

Expression

Bacterial

Mutation

only Jab1 domain (aa 2143-2398)

Promoter

T7

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-LGK/csTEM1

Plasmid

#81164

Purpose

pSALECT plasmid with levoglucosan kinase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase

Tags

Codon swapped TEM-1 beta-lactamase (H26 to W290) …

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAL942

Plasmid

#119721

Purpose

precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C

Tags

none

Expression

Bacterial

Mutation

Alanine 248 to Threonine, Alanine 188 to Cysteine…

Promoter

T7

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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