We narrowed to 149,095 results for: ins;
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Plasmid#171542PurposeEncodes a DGCR8 fragment extending from E502 to the C-terminusDepositorInsertDGCR8 (DGCR8 Human)
ExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO FAPP2-GLTPH
Plasmid#170739PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyExpressionYeastPromoterGAL10Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159825PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertpEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C
Plasmid#81239PurposeU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)
Plasmid#133796PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1 (truncated C-terminal domain only)
TagsNLS(SV40)ExpressionMammalianMutationC-terminal domain onlyPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
Plasmid#133798PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T114A/F115A)
TagsNLS(SV40)ExpressionMammalianMutationT114A/F115APromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD
Plasmid#140305PurposeExpresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-KD
Plasmid#140301PurposeExpresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1942
Plasmid#105867PurposeHigh copy number plasmid containing Ple265 (PCP2 MiniPromoter) insertDepositorInsertPle265
PromoterPle265 (PCP2 MiniPromoter)Available SinceFeb. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-GST-Mps1-KD
Plasmid#114066Purposeprotein productionDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-WT
Plasmid#114065Purposeprotein productionDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
Tags6His-TEVExpressionBacterialMutationR52APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
KCC4_HUMAN_D0
Plasmid#79744PurposeThis plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK10_HUMAN_D0
Plasmid#79722PurposeThis plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-ZKSCAN1 548-847
Plasmid#69885PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in DrosophilaDepositorAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A DH domain
Plasmid#69794PurposePebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH domain
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationContains only the Pebble DH domainPromoterhsp70Available SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZM3[I:FruR-L,I:RbsR-L]
Plasmid#60777PurposeContains PI driving expression FruR-L, and PI driving expression of RbsR-L.DepositorInsertsFruR-L
RbsR-L
MutationLacI/GalR repressor chimera. LacI DBD with FruR L…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (K24N)
Plasmid#62079PurposeExpression of human p53 (1-73) (K24N) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD residues 1-73; K24NPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(219-408)
Plasmid#35067DepositorAvailable SinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
Tags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-PR rep/cap
Plasmid#197565Purposeencodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic deliveryDepositorInsertsAAV Rep genes
AAV9 VP1 Cap gene with PR insert
UseAAVMutationcontains 7mer insert PRPPSTH between amino acids …Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
ExpressionBacterialPromoterPcel and trcAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-XRI-HA
Plasmid#178056PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.DepositorInsertXRI-HA
UseAAVTagsHA-MBPExpressionMammalianPromoterHuman ubiquitin C (UBC) promoterAvailable SinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
TagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP
Plasmid#201681PurposeLentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expressionDepositorInsertsSFTPC promoter region (2kb upstream)
TagRFP
UseLentiviralExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
RpH-ILV-3xALFA
Plasmid#225135PurposeRpH-ILV probe strongly colocalises with lysosomal markers.DepositorInsertTMEM192 (TMEM192 Human)
ExpressionMammalianAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G
Plasmid#194464PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only