We narrowed to 17,271 results for: form
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Plasmid#211784PurposeSunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertSnoopCatcher-KRAB
Tags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
Tags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG5-Myr-FLAG-p110α-DM
Plasmid#55713Purposeeukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227ADepositorInsertp110 alpha (Pik3ca Mouse)
Tagsmyristoylation tag (Myr-FLAG)ExpressionMammalianMutationT207D, K227APromoterT7Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA V14 (CT#846)
Plasmid#13949DepositorAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Luc
Plasmid#25743PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 d80 pET21a+
Plasmid#173045PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
Tags6xHis tagExpressionBacterialMutationwith N-terminal 80 amino acids deletionAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema4f.b-Fc-His
Plasmid#72159PurposeExpresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-Orai1
Plasmid#89818PurposeExpress Orai1 in mammalian cellsDepositorInsertOrai1 (ORAI1 Human)
UseRetroviralAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)‐NeoR
Plasmid#65528PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Plasmid#108848PurposeInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMag,NLSExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C G126
Plasmid#123079PurposeGateway entry clone encoding human MAP1LC3C G126DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneMutationDeleted amino acids 127-147. Stop codon after G12…Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FOXM
Plasmid#67118PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT
Plasmid#164011PurposeMammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
7749 Bicistronic_ires_hygro
Plasmid#64374PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance geneDepositorTypeEmpty backboneUseRetroviralAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Plasmid#108846PurposeInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMag,NLSExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CKAP2-mNeonGreen pHAT
Plasmid#175947PurposeBacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bin1 delta SH3
Plasmid#168283PurposeExpress SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.DepositorAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3C G126
Plasmid#123096PurposeExpresses 3xFLAG-LC3C G126 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
Tags3xFLAGExpressionMammalianMutationDeleted amino acids 127-147. Stop codon after G12…PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
attB‐mCh‐Poly(A)-PuroR
Plasmid#65529PurposePuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CARMIL2
Plasmid#118740PurposeTo express CARMIL2 with Flag tag on N-terminusDepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Plasmid#108845PurposeInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAIExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Plasmid#108843PurposeInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1ExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2E1
Plasmid#67089PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
IX301: pMVP (L3-L2) myc epitope tag + polyA
Plasmid#121753PurposepMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertmyc epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cntn4.2-AP-His
Plasmid#71942PurposeExpresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EBNA1BP2
Plasmid#67072PurposeStandard for cell-free expression benchmarking.DepositorInsertEBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
UseCell-free expressionTagsEGFP and HisPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V E37G
Plasmid#12277DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…Available SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V Y40C
Plasmid#12279DepositorInsertH-Ras G12V Y40C (HRAS Human)
UseRetroviralExpressionMammalianMutationContains G12V activating mutation. The Y40C mutat…Available SinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
(B) HYG marker(R) (SBE264)
Plasmid#195013Purposehygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+HYG+tGPD
ExpressionBacterialPromoterpXYLAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) BLE marker(R) (SBE262)
Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
ExpressionBacterialPromoterpXYLAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA
Plasmid#104984PurposeExpresses ST-Renilla reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-Intu
Plasmid#65430PurposeFor mammalian expression of EGFP-mouse InturnedDepositorAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_3X Flag
Plasmid#194161PurposeExpresses eGFP fluorescent protein with a 3X flag tagDepositorInsertGFP with 3X Flag
Tags3x FlagExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
IX601: pMVP (L3-L2) FLAG epitope tag + polyA
Plasmid#121751PurposepMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertFLAG epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ntn5.a-Fc-His
Plasmid#72107PurposeExpresses the entire Netrin 5, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP no-stop
Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneMutationStop codon removedAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C no-stop
Plasmid#123208PurposeGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneMutationStop codon removedPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-7
Plasmid#14968DepositorInsertc-myc promoter (-26/+334) (MYC Human)
UseLuciferaseExpressionMammalianMutation-26/+334 (relative to the P2 transcription initia…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA N19 (CT#847)
Plasmid#14002DepositorAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116A
Plasmid#123084PurposeGateway entry clone encoding human GABARAPL1 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneMutationGlycine 116 to AlanineAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
KZ901: pMVP (L3-L2) P2A-eGFP + WPRE
Plasmid#121772PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ401: pMVP (L3-L2) P2A-Neo + polyA
Plasmid#121781PurposepMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Neo + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shcIAP1-B
Plasmid#44131DepositorAvailable SinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
KZ601: pMVP (L3-L2) P2A-Zeo + polyA
Plasmid#121783PurposepMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Zeo + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTPTP alpha (S180A/S204A)
Plasmid#17700DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer
Plasmid#86195PurposeU6 based expression of N meningitidis sgRNADepositorInsertnmCas9 sgRNA cloning cassete
UseLentiviralPromoterU6Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only