We narrowed to 12,311 results for: BASE

1. CAG FUCCI

   Plasmid

   #175266

   Purpose

   Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

   Depositor

   James Monaghan

   Article

   Duerr et al Development. 2022 Mar 15;149(6). pii: 27

   Insert

   FUCCI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-DEST-hPROX1-tdTomato-PuroTK

   Plasmid

   #141027

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPRIME-CMV-Neo-recipient

   Plasmid

   #11659

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling Neomycin expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Neo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Blast-miniTurbo

   Plasmid

   #207681

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSP6-EBNA(2A+DBD)

   Plasmid

   #98749

   Purpose

   In vitro transcription of modified EBNA1 mRNA

   Depositor

   Jim Vadolas, Sara Howden

   Article

   Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

   Insert

   Modified EBNA1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGolden-Neo

   Plasmid

   #51422

   Purpose

   Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

   Depositor

   Yonglun Luo

   Article

   Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

   Insert

   PGK1 phosphoglycerate kinase 1 (PGK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSGAb-spe

   Plasmid

   #122000

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid

    #170578

    Purpose

    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL5 (CDKL5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    E854G- please see depositor comments

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRB_SCF2_LD6_CTEV

    Plasmid

    #58879

    Purpose

    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP_SCF2_LD6_NTEV

    Plasmid

    #58878

    Purpose

    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-hSyn-EGFP

    Plasmid

    #114215

    Purpose

    Lentiviral vector (control) that targets expression of EGFP to neurons

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCP-RecT

    Plasmid

    #183827

    Purpose

    pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    MCP-RecT_NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BmTyr-Flag

    Plasmid

    #220779

    Purpose

    non-targeted BmTyr throughout the cytoplasm

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    Bacillus megaterium Tyrosinase
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-KO-Puro

    Plasmid

    #227262

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-GFP(S65T)-TRP1

    Plasmid

    #41597

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (S65T) and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK445 (BSD-P2A-BromoTag)

    Plasmid

    #214375

    Purpose

    BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. P16ΔS:S10-(MCS)

    Plasmid

    #108182

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-VP2SA11

    Plasmid

    #89163

    Purpose

    Express Rotavirus SA11 VP2 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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