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PARP15-c020

Plasmid

#173101

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

N78-S267, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NELFE

Plasmid

#222559

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

NELFE (NELFE Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAID 1.1 N-T2A-Bsr

Plasmid

#105985

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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PARP15-c021

Plasmid

#173102

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

G287-N470, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMT451

Plasmid

#139391

Purpose

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)

Use

Synthetic Biology

Promoter

BA inducible promoters

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-VPS21

Plasmid

#18842

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

VPS21 (VPS21 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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PC1-29-9-2_GAL4-VHL

Plasmid

#200434

Purpose

Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

GAL4-VHL

Tags

GAL4, VHL

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGECInano

Plasmid

#186188

Purpose

AAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicator

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Use

AAV

Promoter

CAG

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_0

Plasmid

#127553

Purpose

Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 0

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS58

Plasmid

#135311

Purpose

A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

HygR-P2A-AID

Use

N terminal tagging plasmid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYX233

Plasmid

#163762

Purpose

Empty vector for Gal-inducible yeast expression

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Type

Empty backbone

Tags

Hemagglutinin (HA) tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p416 46Q GPD

Plasmid

#1178

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 46Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-EZH2

Plasmid

#222528

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

EZH2 (EZH2 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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p426 103Q GPD

Plasmid

#1184

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp2-GFPuv

Plasmid

#139083

Purpose

Low phosphate inducible promoter yibDp2 for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yibDp2-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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