4,178 results

1. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETa mEos2

   Plasmid

   #20341

   Purpose

   bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

   Depositor

   Loren Looger

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   mEos-2
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-nmdCas9-3XmCherry

   Plasmid

   #64110

   Purpose

   Expression of Nm dCas9-3XmCherry in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-Blue FP

   Plasmid

   #52115

   Purpose

   a bicistronic IRES-Blue FP containing retroviral vector

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-Blue FP

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-PSF-WT

   Plasmid

   #35183

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   PSF (SFPQ Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Cre-GFP

   Plasmid

   #68544

   Purpose

   AAV expression of Cre-GFP fusion

   Depositor

   Eric Nestler

   Article

   Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRB-NLuc

   Plasmid

   #31181

   Depositor

   David Piwnica-Worms

   Article

   Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lentiviral-FOP-dGFP-reporter

   Plasmid

   #14885

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   mutant LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB1H2w2-zif268

    Plasmid

    #18045

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268
    

    Expression

    Bacterial

    Mutation

    Zif268 is fused to the omega subunit of RNA polym…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmStrawberry-Atg4BC74A

    Plasmid

    #21076

    Depositor

    Tamotsu Yoshimori

    Article

    Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

    Insert

    Atg4B(C74A) (Atg4b Mouse)
    

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    220-222 TGC(Cys) is changed to GCA (Ala)

    Available Since

    June 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-FNLS-P2A-Puro

    Plasmid

    #110841

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. WWP-Luc (p21/WAF1 promoter)

    Plasmid

    #16451

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-gRNA

    Plasmid

    #46759

    Purpose

    gRNA cloning vector for in vitro transcription of target gRNA

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    gRNA core
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Casp3 C163A-myc

    Plasmid

    #11814

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 catalytic mutant (CASP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C163A

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCardinal-C1

    Plasmid

    #54799

    Purpose

    Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCS2 Flag-JunB

    Plasmid

    #29687

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    jun B oncogene (JUNB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-Ago2

    Plasmid

    #21538

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    The first 7aa of this Ago2 protein are different …

    Available Since

    Sept. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104495

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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