4,142 results

1. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR5'_ubi:loxP-EGFP-loxP

   Plasmid

   #27322

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   ubiquitin:loxP-EGFP-loxP
   

   Use

   Multisite gateway 5' entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. hAur-A

   Plasmid

   #8510

   Depositor

   Joan Ruderman

   Article

   Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

   Insert

   hAur-A (AURKA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgRNA-modified

   Plasmid

   #84477

   Purpose

   guide RNA expression lentiviral construct

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   guide RNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-RIPK1

   Plasmid

   #78842

   Purpose

   To overexpress RIPK1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP FAK

   Plasmid

   #50515

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-gRNA

   Plasmid

   #46759

   Purpose

   gRNA cloning vector for in vitro transcription of target gRNA

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   gRNA core
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFP-zyxin

   Plasmid

   #26720

   Depositor

   Anna Huttenlocher

   Article

   Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

   Insert

   Zyxin (ZYX unknown, Human)
   

   Use

   Tags

   RFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-SEP-NRI

    Plasmid

    #23999

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    NR1 signal sequence (aa1-20) and SEP (supereclipt…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL hygro H-Ras V12

    Plasmid

    #18749

    Depositor

    Scott Lowe

    Article

    Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRV.GFP FOXP3

    Plasmid

    #13250

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmAmetrine-DEVD-tdTomato

    Plasmid

    #18879

    Purpose

    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

    Depositor

    Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Insert

    mAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP14

    Plasmid

    #22569

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP14 (USP14 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA-mTmG

    Plasmid

    #26123

    Depositor

    Liqun Luo

    Insert

    Precursor mT/mG
    

    Use

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge