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We narrowed to 15,050 results for: NOL

Showing: 8051 - 8100 of 15050 results

  1. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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  12. pK1E-RNAP

    Plasmid
    #48144
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase K1E
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48144-plasmid-map-sequence-id-69927

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  13. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  17. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  26. pEGFP-C1-M3(T3D)471-721

    Plasmid
    #56657
    Purpose
    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing
    Depositor
    Max Nibert
    Article
    Broering et al J Virol. 2005 May;79(10):6194-206.
    Insert
    M3(T3D)421-721
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    insert encodes aa 471-721 + native stop codon of …
    Promoter
    CMV-IE
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    56657-plasmid-map-sequence-id-87642

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  32. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  38. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  47. pCosSAD1AprR

    Plasmid
    #190786
    Purpose
    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination
    Depositor
    Jan Kormanec
    Article
    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(
    Inserts
    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190786-plasmid-map-sequence-id-375582

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  48. pOCC119

    Plasmid
    #118884
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mGFP, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118884-plasmid-map-sequence-id-232361

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Showing: 8051 - 8100 of 15050 results