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Showing: 8051 - 8100 of 18165 results

  2. Flag-APEX2-C Cloning Vector

    Plasmid
    #128146
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128146-plasmid-map-sequence-id-248610

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  7. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91788-plasmid-map-sequence-id-180614

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  9. pXR004_puroR

    Plasmid
    #219820
    Purpose
    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219820-plasmid-map-sequence-id-441123

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  11. pGL003

    Plasmid
    #215335
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pSpank
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215335-plasmid-map-sequence-id-426095

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  12. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  14. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  15. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  18. pExp-DsbA

    Plasmid
    #112562
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbA(AA20-208) and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112562-plasmid-map-sequence-id-217223

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  20. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  21. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220982-plasmid-map-sequence-id-437118

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  22. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220983-plasmid-map-sequence-id-437500

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  24. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

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  26. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  27. pQC V5 MCS IRES Puro

    Plasmid
    #110342
    Purpose
    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110342-plasmid-map-sequence-id-218292

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  30. pMH-EBFP

    Plasmid
    #161714
    Purpose
    Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    161714-plasmid-map-sequence-id-319502

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  31. pAttPF3Hyg

    Plasmid
    #244929
    Purpose
    attP Hyg Resistant plasmid for RMI targeting an attB site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244929-plasmid-map-sequence-id-486014

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  33. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  40. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

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  43. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid
    #51656
    Purpose
    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange
    Depositor
    Adam Cohen
    Insert
    Quasar2-mOrange-Chr64-GFP
    Use
    Lentiviral
    Tags
    GFP and mOrange
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    March 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    51656-plasmid-map-sequence-id-319739

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  44. pInt_attP1_tsXis_sacB_kanR

    Plasmid
    #205302
    Purpose
    Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205302-plasmid-map-sequence-id-404790

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  46. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

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  48. pHEE-R-bar9si

    Plasmid
    #222834
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222834-plasmid-map-sequence-id-446485

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  49. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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  50. pHEE-R-hyg5A9si

    Plasmid
    #222835
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222835-plasmid-map-sequence-id-446486

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Showing: 8051 - 8100 of 18165 results