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pLenti-BE3-P2A-GFP-PGK-Puro

Plasmid

#110867

Purpose

Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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LAMP1-SBP-GFP

Plasmid

#120172

Purpose

Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Lysosomal-associated membrane protein 1 (Lamp1 Rat)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154786

Purpose

Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Synthetic, Fly)

Use

Lentiviral

Tags

GFP, V5, and mCherry

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple

Plasmid

#214417

Purpose

Peroxisome localization of PMP34 fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34 (SLC25A17 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pNA 3C myc scfvFLAG

Plasmid

#234990

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvFLAG

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Kif5A-GFP-CIBN

Plasmid

#102252

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC

Plasmid

#200558

Purpose

Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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HsTPC2-EYFP

Plasmid

#135194

Purpose

Expresses tagged TPC2 in mammalian cells

Depositor

Antony Galione

Article

Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

Insert

TPC2 (TPCN2 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TCF21

Plasmid

#192894

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TCF21 (TCF21 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-Miro1TM

Plasmid

#102247

Purpose

Expresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

CRY2PHR-mCherry-Miro1TM (RHOT1, CRY2 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCMVNeoDEST(705-1)-hAXL

Plasmid

#124796

Purpose

Expresses AXL in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mouse Gfi1-GFP

Plasmid

#91892

Purpose

3rd generation lentiviral expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

Unknown

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-acta2

Plasmid

#78686

Purpose

acta2 promoter for Gateway cloning

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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