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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pGD425

Plasmid

#132381

Purpose

contains the sequence of mTurquoise2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mTurquoise2

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-30-mCherry

Plasmid

#234061

Purpose

Encodes a Melt variant with a switching temperature of 30°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-30-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA11 1397N

Plasmid

#221935

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6-11 1397C

Plasmid

#221938

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6 1397N

Plasmid

#221939

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA11 1397N

Plasmid

#221940

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pZS47a luxI-laa RBS

Plasmid

#48892

Purpose

ArsR-regulated expression of luxI-laa

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pArsR

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pHES835

Plasmid

#87946

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Promoter

pZ

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHyg-N1

Plasmid

#131852

Purpose

This is the empty vector control for the mBanana competition experiment

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3866

Plasmid

#185743

Purpose

Sub-array backbone 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1019_Tier3(PB)

Plasmid

#169649

Purpose

Tier-3 vector for stable integration of up to three cassettes via piggyBac transposase (ITR-A1::A2::A3-ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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EC10161

Plasmid

#154060

Purpose

Level 0.5 Golden Gate backbone, containing loxP sites.

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZE31-DsRed-ms2x2

Plasmid

#26248

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

ms2 binding sites (x2)

Expression

Bacterial

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA

Plasmid

#21302

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

lambdaN-HA

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCdelta.RNAi

Plasmid

#10819

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC delta RNAi (PRKCD Human)

Use

RNAi

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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