165,947 results

1. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

   Plasmid

   #196847

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

   Plasmid

   #196828

   Purpose

   Express dpdpCas13b with AAAA mutation

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKB238

   Plasmid

   #170033

   Purpose

   Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Neuropeptide Y (NPY Synthetic, Human, Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Promoter

   Ptac and PvirK

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_RAPGEF4_DEP

   Plasmid

   #109895

   Purpose

   Protein expression and purification of RAPGEF4_DEP

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RAPGEF4_DEP (RAPGEF4 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6379 H10-K218T

   Plasmid

   #1250

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K218T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K218T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. 6380 H10-K620T

   Plasmid

   #1251

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K620T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K620T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Promoter

   tac and unknown

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDM-Hgpm2

    Plasmid

    #164441

    Purpose

    Encodes codon-optimized HIV gagpol for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    Codon Optimized HIV-1 GagPol
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mfn2-Myc

    Plasmid

    #23213

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn2 (Mfn2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. JD150

    Plasmid

    #229485

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, E4orf6, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Expression

    Mammalian

    Promoter

    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mRuby3-C1

    Plasmid

    #127808

    Purpose

    mRuby3 C1 Cloning vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mRuby3
    

    Expression

    Mammalian

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-CD36-C-10

    Plasmid

    #55011

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF5B-FRT-GFP-αTAT1[D157N]

    Plasmid

    #27100

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Tags

    GFP and S-tag

    Expression

    Mammalian

    Mutation

    D157N

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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