Addgene

We narrowed to 20,931 results for: Tes

Showing: 4861 - 4890 of 20931 results

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pMP119

Plasmid

#207949

Purpose

GreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Syndecan-1 shRNA (ORF)

Plasmid

#40622

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(ORF) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-22

Plasmid

#64229

Purpose

Expresses miR-22 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR22 (MIR22, MIR22HG Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUdO4t

Plasmid

#210249

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Use

Tags

FRB (T2098L)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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P-miR-145 (pTransluc-miR-145-1)

Plasmid

#21496

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

1.5kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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Talin Head

Plasmid

#186140

Purpose

For expression of recombinant Talin head domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

TLN1 (TLN1 Human)

Use

Tags

His6 (tev cleavage)

Expression

Bacterial

Mutation

Promoter

Lac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKE014

Plasmid

#207908

Purpose

GG entry for zCas9i expression

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

SpCas9_introns_2xNLS-Pea3A(T) term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ237

Plasmid

#212320

Purpose

Express KnFnuc

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ226

Plasmid

#212316

Purpose

Express DpFnuc with pureexpress

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-Rap1b N17

Plasmid

#118322

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b N17 (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

S17N

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-MYB-3'UTR

Plasmid

#25798

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

3'UTR of human MYB (MYB Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

ppag promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Tags

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMP107

Plasmid

#207925

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Use

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pTNS2

Plasmid

#87802

Purpose

For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

Depositor

Yu-Zhu Zhang

Article

Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

Type

Empty backbone

Use

Tags

6xHis and Strep II

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

3.6TPH

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pET15b-hnRNP-F

Plasmid

#23021

Depositor

Douglas Black

Article

Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

Insert

hnRNP-F (HNRNPF Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAM750

Plasmid

#207889

Purpose

Level 0 for A3 module (5' TCCC-3' AACA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM749

Plasmid

#207888

Purpose

Level 0 for AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM758

Plasmid

#207897

Purpose

Level 0 for C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM756

Plasmid

#207895

Purpose

Level 0 for C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM757

Plasmid

#207896

Purpose

Level 0 for C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM745

Plasmid

#207887

Purpose

Level 0 for A1 module (5' ACCT-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge