We narrowed to 10,838 results for: ENA

1. pET28-sfGFP[N150TAA]

   Plasmid

   #164581

   Purpose

   superfolder GFP with N150TAA mutation

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Insert

   sfGFP-150TAA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N150TAA Mutation

   Promoter

   T7

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-zfCSF3b

   Plasmid

   #65613

   Purpose

   express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pJMP1363

   Plasmid

   #119279

   Purpose

   for stabilizing ICE in the presence of rapI expression

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

   Plasmid

   #164262

   Purpose

   Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

   Depositor

   Joseph Peters

   Article

   Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

   Insert

   Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRvCAHS3-mEGFP

   Plasmid

   #205008

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRvACT-mCherry

   Plasmid

   #205002

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro-mIR-31 sponge

   Plasmid

   #22694

   Purpose

   Stable suppression of miR-31 function

   Depositor

   Bob Weinberg

   Article

   Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

   Insert

   miR-31 (MIR31 Human)
   

   Use

   Retroviral

   Tags

   *

   Expression

   Mammalian

   Mutation

   miR-31 sponge with 7x tandem binding sites to the…

   Promoter

   Available since

   March 11, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pACE-GIRK4-S

   Plasmid

   #172425

   Purpose

   Expression of full-length, human GIRK4 with C-terminal StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN0606 (pDEST-DHFR F[3] N-term,HygR)

   Plasmid

   #210488

   Purpose

   pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Destination vector for gateway…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

    Plasmid

    #210485

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

    Plasmid

    #210487

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKB12 (pDEST-DHFR F[3] C-term, HygR)

    Plasmid

    #210486

    Purpose

    pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-ZZ-TEV-SNAPf-BICD2N(1-400)

    Plasmid

    #111857

    Purpose

    N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICD2N(1-400)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH2523

    Plasmid

    #191358

    Purpose

    Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP3
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1171

    Plasmid

    #119253

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1170

    Plasmid

    #119252

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_A0402

    Plasmid

    #91009

    Purpose

    Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    AtUbi10

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXWJ109HgAmp30E

    Plasmid

    #123149

    Purpose

    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SauCas9 EMX1-nicking sgRNA

    Plasmid

    #169860

    Purpose

    SauCas9 nicking sgRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9 EMX1-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSilencer2.1-U6-4JC1RR

    Plasmid

    #190847

    Purpose

    For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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