Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 27,291 results for: Tat

Showing: 3261 - 3280 of 27291 results

  10. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207357-plasmid-map-sequence-id-408418

    Enlarge

  14. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118717-plasmid-map-sequence-id-230455

    Enlarge

  15. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

    Plasmid
    #187732
    Purpose
    Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    deletion of residues 1-410 and 718-1959 and 1997-…
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187732-plasmid-map-sequence-id-372456

    Enlarge

  17. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181855
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181855-plasmid-map-sequence-id-362798

    Enlarge

  18. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181854
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181854-plasmid-map-sequence-id-362794

    Enlarge

  19. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

    Enlarge

  20. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

    Enlarge

Showing: 3261 - 3280 of 27291 results