We narrowed to 6,927 results for: 11
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Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
UseTagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.A184V
Plasmid#106181PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterhSynapsinAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)
Plasmid#65446PurposeExpression of ephrinB1 and RFP in mammalian cellsDepositorInsertephrin B1 (Efnb1 Mouse)
UseLentiviralTagsSV40-RFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.A184S
Plasmid#106201PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3
Plasmid#106204PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184S + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB2-SV40-GFP (B2G)
Plasmid#65442PurposeExpression of mouse EPHB2 and GFP in mammalian cellsDepositorInsertEPHB2 (Ephb2 Mouse)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationSee depositor comments below.PromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL WT
Plasmid#107159PurposeFor mammalian expression of Flag-hPKL WTDepositorInsertFlag-hPKL WT (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S
Plasmid#106177PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]
Plasmid#122102PurposeAAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-GFP
Plasmid#122100PurposeAAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertGFP
UseAAVTagsNAExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceMay 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS010: pLX304-CheRiff-GFP
Plasmid#62986PurposeLentivirus production of CheRiff channelrhodopsin actuatorDepositorInsertCheRiff-eGFP
UseLentiviral; Gateway destination vectorTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKM2 WT
Plasmid#107158PurposeMBP-rPKM2 WT for production and purification of rat PKM2 WTDepositorInsertMBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3/hArf6(WT)-EGFP
Plasmid#79423PurposeExpresses C-terminally EGFP-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_ISG15
Plasmid#99322PurposeLuciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr1: 947976 -949995 (ISG15 Human)
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3
Plasmid#106206PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72A + mRuby3 fusionPromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Chronos-tdTomato]
Plasmid#105834PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLXSN p200 CUX1
Plasmid#90470PurposeRetroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectivelyDepositorInsertCUX1 (CUX1 Human)
UseRetroviralTagsHa and mycExpressionMammalianMutationPromoterMoloney murine leukemia virus long terminal repeatAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1 double gRNAs
Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorInsertMultiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.A184V
Plasmid#106202PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorUseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:IL2_SigP:NLuc
Plasmid#197266PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertInterleukin-2 homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FLEX-rc[Chronos-GFP]
Plasmid#62725PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1αAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)
Plasmid#114094PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (S542R/K607R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationS542R and K607RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fgf4enhLV (FpG12)
Plasmid#69444Purposepositive control plasmid for lentiviral reporter expression in ESCsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.S72A
Plasmid#106203PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S
Plasmid#106183PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.A184S
Plasmid#106195PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterGFAPAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only