4,082 results

1. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV JMJD3 mutant

   Plasmid

   #21214

   Depositor

   Paul Khavari

   Article

   Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1390A

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PTEN

   Plasmid

   #13039

   Depositor

   Alonzo Ross

   Article

   Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVpuro-CMV-N-EGFP

   Plasmid

   #122848

   Purpose

   N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKOV

   Plasmid

   #25769

   Depositor

   George Church

   Article

   Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ920

   Plasmid

   #42234

   Depositor

   Jennifer Doudna

   Article

   Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GFP, HA epitope, and NLS (nuclear localization si…

   Expression

   Mammalian

   Mutation

   codon-optimized synthetic DNA sequence

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-puro-cMyc

   Plasmid

   #46970

   Purpose

   lentiviral expression of c-Myc

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-IRES-Thy1.1 DEST

   Plasmid

   #17442

   Purpose

   Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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