We narrowed to 12,214 results for: LEA

Showing: 3321 - 3340 of 12214 results

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pPS2035

Plasmid

#1368

Depositor

Pamela Silver

Publication

Brodsky et al RNA 2000 Dec;6(12):1737-49.

Insert

U1A (1-94) (RNU1-4 Human)

Tags

GFP

Expression

Yeast

Mutation

U1A (1-94)

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

Plasmid

#217808

Purpose

Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

Depositor

Benjamin Kleinstiver

Publication

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Puro

Plasmid

#169220

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Puro

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Blast

Plasmid

#169221

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Blast

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Publication

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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KAT14-pDEST10

Plasmid

#118382

Purpose

HIS-tagged insect cell expression vector with KAT14.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Tags

HIS

Expression

Insect

Promoter

Polyhedrin

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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NES-iRFP-P4Mx1

Plasmid

#108144

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1505

Plasmid

#29300

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ple34 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SuExp-hPLD3

Plasmid

#201243

Purpose

express full length human PLD3 protein

Depositor

David Nemazee

Publication

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLD3 (PLD3 Human)

Expression

Mammalian

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA6

Plasmid

#179680

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR

Expression

Mammalian

Mutation

Q1310R, S1330I, T1380I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Publication

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRG705_lexO-AsiSI_LexA-TF_LEU2MX

Plasmid

#154817

Purpose

Inducible expression of AsiSI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Publication

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AsiSI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Publication

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Publication

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Expression

Mammalian

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 K52R

Plasmid

#118298

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Publication

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERK2 K52R (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

K52R

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RHBDD1 (RHBDD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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3xFNLDD/pMXs-IG

Plasmid

#48972

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Publication

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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