We narrowed to 15,769 results for: LEA

1. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT3928

   Plasmid

   #113331

   Purpose

   control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Use

   Unspecified

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUNIV-EGFP

   Plasmid

   #24706

   Depositor

   Cynthia Czajkowski

   Article

   Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

   Insert

   EGFP (eGFP Aequoria victoria)
   

   Expression

   Bacterial, Mammalian, and Y…

   Available Since

   Dec. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

   Plasmid

   #205278

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pChimera

   Plasmid

   #61476

   Purpose

   Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   U6-26:sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6-26

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB660-CYCD3;1

   Plasmid

   #140751

   Purpose

   Inducing plant cells to enter mitosis

   Depositor

   Bo Liu

   Article

   Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

   Insert

   Cyclin D3;1 (CYCD3;1 Mustard Weed)
   

   Tags

   TagRFP

   Expression

   Plant

   Promoter

   CaMV 35 promoter

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28_Caspase-4_K356D

   Plasmid

   #214312

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K356D

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH726_pJH_LldR_PlldR-sfGFP.15TAG

    Plasmid

    #163730

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.15TAG
    

    Expression

    Bacterial

    Mutation

    gfp with first 15 leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH773_pTech_PlldR-HHRz-supP(opal)

    Plasmid

    #163732

    Purpose

    Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    hhrz-supP
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG.CenpB(FL)-GFP-Halo

    Plasmid

    #176205

    Purpose

    Centromeric anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    CenpB-GFP-Halo
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. KBB460

    Plasmid

    #185094

    Purpose

    NMA111-GFP wild type under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111-GFP

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+MT-hToca-1-wt

    Plasmid

    #33030

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Csy4H29AS50C

    Plasmid

    #45029

    Depositor

    Jennifer Doudna

    Article

    Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

    Insert

    Csy4H29AS50C
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    H29A mutation to abolish catalytic activity and S…

    Promoter

    T7

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pMJ824

    Plasmid

    #39314

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ333: pU6-MmeFz2_omegaRNA-entry

    Plasmid

    #205267

    Purpose

    Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG-Cherry-N1-PRKAG1

    Plasmid

    #30308

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase γ1 (PRKAA1 Human)
    

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pL0M-S-mNeonGreen-EC18153

    Plasmid

    #137075

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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