We narrowed to 6,212 results for: cas9 expression plasmid
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Plasmid#64157PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_GAL4UAS-Luciferase reporter
Plasmid#64158PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA2 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-spe
Plasmid#122000PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN-sacB
Plasmid#196249PurposePlasmid containing the sacB geneDepositorInsertattR2-sacB-attL3
UseTagsExpressionBacterialMutationPromotersacB promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.dTomato
Plasmid#89395PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS423-SgH
Plasmid#163972PurposeHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-Esp3I
Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWR91
Plasmid#203915Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locusDepositorInsertCRF1 plus flanking (CRF1 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR90
Plasmid#203914Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locusDepositorInsertGSC2 plus flanking (GSC2 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR89
Plasmid#203423Purposeempty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locusDepositorInsertEGC3 plus flanking (EGC3 S. stipitis (yeast))
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYMCR2_Gb_PMLTSS
Plasmid#140285PurposeCRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-inDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHEE401E
Plasmid#71287PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only