We narrowed to 10,377 results for: plasmids 101

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pAAV-U6-DR30(SapI)-CMV-intron-GFP-pA

Plasmid

#192493

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-T2A-mCherry-pA

Plasmid

#192497

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGgaatacattaccaacGTGGTGtacGTG

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-HA-mCherry-pA

Plasmid

#233028

Purpose

To Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

Plasmid

#233031

Purpose

To express a DjCas13d-compatible gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-compatible gRNA expression cassette

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-hfCas13d-pA

Plasmid

#195866

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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gh107

Plasmid

#106777

Purpose

expression of gRNA targeting B3GALT4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT4 (B3GALT4 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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gh82

Plasmid

#106753

Purpose

expression of gRNA targeting MGAT4D

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT4D (LOC152586 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60229

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Has Service

AAV8

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

Plasmid

#170196

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 D614G Spike

Plasmid

#185692

Purpose

Expresses codon-optimized full length SARS-CoV-2 D614G Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 D614G Spike (S )

Expression

Mammalian

Mutation

Contains the following mutation: D614G

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET11a_3xFlag-nsp14 (SARS-CoV-2)

Plasmid

#169159

Purpose

To express SARS-CoV-2 nsp14 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)

Tags

3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

Plasmid

#197504

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-ABE8e-NRCH-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-PuroR [M1G]

Plasmid

#171809

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Htt171-18Q-myc-WPRE

Plasmid

#107936

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Article

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of wild type HTT (HTT Human)

Use

AAV

Promoter

hSynapsin

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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