We narrowed to 10,318 results for: plasmids 101

1. pGL3-noSV40-EC1.45-human_min1-mouse_min2

   Plasmid

   #173965

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Human minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-noSV40-EC1.45-human_min1-lizard_min2

   Plasmid

   #173969

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Human minimal enhancer region 1 (min1) and lizard minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Spike Display_Part 5 Spacer

   Plasmid

   #172733

   Purpose

   Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. Spike Display_Part 4 Spacer

   Plasmid

   #172732

   Purpose

   Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Spike Display_Part 3 Spacer

   Plasmid

   #172731

   Purpose

   Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. Spike Display_Part 1 Spacer

   Plasmid

   #172729

   Purpose

   Encodes Part 1 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. Spike Display_Part 1b Spacer

   Plasmid

   #172728

   Purpose

   Encodes Part 1b of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Spike Display_Part 1a Spacer

   Plasmid

   #172727

   Purpose

   Encodes Part 1a of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist-SARS-CoV-2 Spike-deltaC18 AY.4.2

    Plasmid

    #212457

    Purpose

    Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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