58,080 results

1. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

   Plasmid

   #65417

   Purpose

   AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Has Service

   AAV8

   Insert

   hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HSPB1

   Plasmid

   #155670

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   HSPB1 (HSPB1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX SHP-2(NC)-SH2

   Plasmid

   #46499

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(NC) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-222

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP GFP-Stx17 WT

   Plasmid

   #45909

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   Syntaxin 17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2861

   Plasmid

   #143644

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-FLAG-MLL-AF9

   Plasmid

   #71444

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-AF9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SMG6

   Plasmid

   #156020

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SMG6 (SMG6 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CALCOCO1-HA

   Plasmid

   #137766

   Purpose

   Expression and localization of tagged CALCOCO1

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 (CALCOCO1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-TOMM20-N-10

    Plasmid

    #54282

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3-HA-RAPGEF2

    Plasmid

    #110162

    Purpose

    Expression of human RAPGEF2 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

    Insert

    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPTN-EGFP

    Plasmid

    #27052

    Depositor

    Beatrice Yue

    Article

    Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-BAZ1A

    Plasmid

    #65371

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1A (BAZ1A Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    796I compared to all reference sequences

    Promoter

    CMV

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. tetO-ASCL1-LMX1b-NURR1-PuroR

    Plasmid

    #182298

    Purpose

    Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

    Depositor

    Kristen Brennand

    Article

    Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

    Insert

    ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK EGFP

    Plasmid

    #216110

    Purpose

    PGK EGFP

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    PGK promoter
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid

    #175523

    Purpose

    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag11 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IB-mGFP-FTH1

    Plasmid

    #192800

    Purpose

    Stable expression of mGFP-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    monomeric EGFP (A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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