We narrowed to 4,940 results for: puc

1. pJBL728

   Plasmid

   #140398

   Purpose

   Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-csoO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

   Plasmid

   #204497

   Purpose

   Expresses rtTA and Neomycin resistance genes in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   rtTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMGS58

   Plasmid

   #135311

   Purpose

   A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   HygR-P2A-AID
   

   Use

   N terminal tagging plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL716

   Plasmid

   #140386

   Purpose

   Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-mphO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADH99

   Plasmid

   #90979

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   US_HIS1
   FRT
   pENO1
   C. albicans Cas9
   pMAL2
   FLP
   NAT 1 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PU6::unc-119_sgRNA

   Plasmid

   #46169

   Purpose

   unc-119 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   unc-119 targeting sgRNA (unc-119 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Aurli_150841_GZG

   Plasmid

   #162563

   Purpose

   Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

   Depositor

   Jackie Collier

   Article

   Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

   Insert

   shble
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   GAPDH

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcCAST-sgRNA_entry (CJT83)

   Plasmid

   #181785

   Purpose

   Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAID5XY

   Plasmid

   #165051

   Purpose

   Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AX53_pS.Donor.S1.TS

    Plasmid

    #100290

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS29: pDonor(PmcCAST)

    Plasmid

    #168162

    Purpose

    PmcCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST right end (RE)
    PmcCAST left end (LE)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE656

    Plasmid

    #153254

    Purpose

    M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTX1-SMN1-exon7-ESE1

    Plasmid

    #126041

    Purpose

    In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN1 exon7 ESE1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE474

    Plasmid

    #153265

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE475

    Plasmid

    #153266

    Purpose

    M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. hOC-GFPtpz

    Plasmid

    #110207

    Purpose

    Expresses GFP in mature bone cells.

    Depositor

    David Rowe

    Article

    Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

    Insert

    eGFPtopaz
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Human osteocalcin (BGLAP)

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIIS18-BsaI

    Plasmid

    #128649

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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