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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-SypHluorin 4x (S4x)

Plasmid

#37005

Depositor

Stephen Heinemann, Yongling Zhu

Publication

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SypHluorin 4x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Four copies of pHluorin inserted at site 184-185

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HA-AMOT p130

Plasmid

#32821

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Purpose

Gateway entry vector containing ubi:loxP-EGFP-loxP. Used to assemble ubi:Switch construct.

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Publication

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLM-vexGFP-Oct4

Plasmid

#22240

Purpose

2nd generation lentiviral vector

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Publication

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pBiFC-bFOSDelataZipVC155

Plasmid

#22014

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bFosDelataZIPVC155

Tags

HA

Expression

Mammalian

Mutation

deletion of residues 179-193 in bFos(118-211)

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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RNT1-GFP

Plasmid

#17708

Depositor

Hal Dietz

Publication

Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rent1 (UPF1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Expression

Bacterial

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Publication

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

HA

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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2X PRE TK luc

Plasmid

#11350

Depositor

Donald McDonnell

Publication

Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PRE

Use

Luciferase

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Publication

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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rAAV2/MG1.1

Plasmid

#184540

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.1 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A587L, Q588M; inse…

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mCherry-EGFP-LC3B

Plasmid

#170446

Purpose

Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

Depositor

Sang-Hun Lee

Publication

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

mCherry-GFP-LC3 (MAP1LC3B Human)

Use

Lentiviral

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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