159,332 results

1. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiFC-VN173

   Plasmid

   #22010

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VN173
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Venus residus 1-172

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-LaminA-C-18

   Plasmid

   #55068

   Purpose

   Localization: Nuclear Envelope, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDESTpK7WG2mito

   Plasmid

   #158726

   Purpose

   Plant mitoTALEN vector assembly, Destination vector

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   mitochondrial presequence in front of attR1 in pk7WG2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIRECT_22C

   Plasmid

   #91135

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-hygro-STING

   Plasmid

   #102598

   Purpose

   Express STING for IFN-beta luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Bak

   Plasmid

   #32564

   Depositor

   Richard Youle

   Article

   Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

   Insert

   human Bak (BAK1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP12157 - pAAV-AiE0395h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2157)

    Plasmid

    #208146

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLAG AXL

    Plasmid

    #105933

    Purpose

    Mammalian expression of Flag-tagged AXL

    Depositor

    Rosa Marina Melillo

    Article

    Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

    Insert

    AXL (AXL Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBWB162

    Plasmid

    #140634

    Purpose

    pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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