We narrowed to 841 results for: nanobodies

1. pNIR-Fb59H10

   Plasmid

   #184682

   Purpose

   NIR-Fb to HIV p24

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb59H10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG02583

   Plasmid

   #104129

   Purpose

   Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   NEDD8-specific protease 1 (NEDP1)
   

   Use

   Tags

   14x Histidine tag, Maltose binding protein, and S…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_EGFP_NbALFA

   Plasmid

   #136626

   Purpose

   Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CaaX(Hras)

   Plasmid

   #108886

   Purpose

   Expression of membrane localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   mCherry-CaaX(Hras)
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIR-FbALFA

   Plasmid

   #184678

   Purpose

   NIR-Fb to ALFA-tag

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbALFA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. nls-mCherry

   Plasmid

   #108881

   Purpose

   Expression of nuclear localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   nls-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-dGBP1-TagBFP

   Plasmid

   #80086

   Purpose

   GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   dGBP1-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRE-tdTomato

   Plasmid

   #50798

   Purpose

   Expression of tdTomato under the control of the TRE-promoter

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   TREtight driven tdTomato fluorescent protein
   

   Use

   Mutli-species

   Tags

   Expression

   Mutation

   Promoter

   TREtight

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

    Plasmid

    #162230

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.0_mitoSNAP

    Plasmid

    #136623

    Purpose

    Plasmid encoding outer mitochondrial localization SNAP-Tag

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3E-APEX2-P2A-EGFP

    Plasmid

    #108896

    Purpose

    Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2-P2A-EGFP
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H2B-mCherry

    Plasmid

    #108880

    Purpose

    Expression of human histone2B fused to mCherry (nuclear marker)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    H2B-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBTSG

    Plasmid

    #159420

    Purpose

    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIR-Fbactin

    Plasmid

    #184677

    Purpose

    NIR-Fb to actin

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fbactin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUAS-Cre

    Plasmid

    #50797

    Purpose

    Expression of Cre recombinase under the control of the UAS-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Cre recombinase
    

    Use

    Multi-species

    Tags

    Expression

    Mutation

    Promoter

    UAS-hsp70

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETSG

    Plasmid

    #159418

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Flp-DOG

    Plasmid

    #80084

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    Flp-DOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+_NLS-Tir1-HA

    Plasmid

    #117716

    Purpose

    mRNA synthesis of NLS-TIR1-HA for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBiex-1 Myosin VI HMM_eGFP

    Plasmid

    #82714

    Purpose

    Expresses Myosin VI HMM with a C-terminal eGFP.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Myosin VI
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOPINVHH_BAP_his

    Plasmid

    #210402

    Purpose

    For expression of VHH with C-terminal biotin acceptor peptide and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    biotin acceptor peptide-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOPINVHH_his

    Plasmid

    #210405

    Purpose

    For expression of VHH with C-terminal his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDX_init containing Sb-loop

    Plasmid

    #132697

    Purpose

    pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_loop
    

    Use

    M13 phagmid vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-EGFP-P2A-APEX2-NS

    Plasmid

    #108895

    Purpose

    Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pB2AR-miRFP670nano3

    Plasmid

    #184671

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    B2AR-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOPINVHH_his_myc

    Plasmid

    #210526

    Purpose

    For expression of VHH with C-terminal his and myc tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    His6-myc

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge