We narrowed to 1,593 results for: PTS

1. pHR-YTHDC1-FL-mCherry-Cry2

   Plasmid

   #177124

   Purpose

   Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

   Plasmid

   #177120

   Purpose

   dCasRx mediated RNA tethering of FTO

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   dCasRx-FTO
   

   Use

   Lentiviral

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   dCasRX contains R239A/H244A/R858A/H863A mutations…

   Promoter

   Ef1a

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-IDR1-mCherry-Cry2

   Plasmid

   #177125

   Purpose

   Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (IDR1 domain) (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   IDR1 domain only

   Promoter

   SFFV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_ZKSCAN1_Tau10-12K317M

   Plasmid

   #194164

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT (MAPT Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-YTH-mCherry-Cry2

   Plasmid

   #177126

   Purpose

   Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH domain) (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   YTH domain only

   Promoter

   SFFV

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HI.HslU.RSETB.fl.wt

   Plasmid

   #12556

   Depositor

   David McKay

   Article

   Trame et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   Haemophilus influenzae heat shock locus U
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTXBI-mclover-YTHDC1-FL

   Plasmid

   #177121

   Purpose

   Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSF_his6_sumo_hRNMT_RAM

    Plasmid

    #213528

    Purpose

    Expresses the human RNMT-RAM complex

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Inserts

    human RNMT (RNMT Human)
    RAM
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTXBI-mClover-YTH domain

    Plasmid

    #177123

    Purpose

    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH domain) (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH domain only (IDR1 and IDR2 deletion)

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTXBI-mclover-YTH-IDR2

    Plasmid

    #177122

    Purpose

    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH and IDR2 domains only (IDR1 deletion)

    Promoter

    T7

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pShuttle-FEN1mED

    Plasmid

    #35033

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1mWT
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PT MUTANT E160D

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pffDronpa2F-peroxisome

    Plasmid

    #214608

    Purpose

    Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

    Depositor

    Peter Dedecker

    Article

    Valenta et al Talanta. 2024 Mar

    Insert

    ffDronpa2F-peroxisome
    

    Use

    Tags

    peroxisome targeting sequence (PTS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSKB3.EG10680

    Plasmid

    #11436

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    OsmC (osmC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

    Plasmid

    #213522

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed V290 to A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

    Plasmid

    #213523

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed N306 to K

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

    Plasmid

    #213524

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to S

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

    Plasmid

    #213525

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to H

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

    Plasmid

    #213526

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed H424 to R

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS_KS_attB2_SA(1)_T2ALexA

    Plasmid

    #125221

    Purpose

    Artificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    LexA
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBS_KS_attB2_SA(0)_T2ALexA

    Plasmid

    #125220

    Purpose

    Artificial phase 0 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    LexA
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS_KS_attB2_SA(2)_T2ALexA

    Plasmid

    #125222

    Purpose

    Artificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    LexA
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-22b(+)-UTPase

    Plasmid

    #12667

    Depositor

    SeWon Suh

    Article

    Han et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    dUTP pyrophosphatase (DUT1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK438

    Plasmid

    #71590

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBS_KS_attB2_SA(0)_T2AGeneSwitch

    Plasmid

    #125214

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-22b(+)-RNase HIII

    Plasmid

    #12683

    Depositor

    SeWon Suh

    Article

    Kwak et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    RNase HIII
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EF2451_pET21a(+)

    Plasmid

    #13840

    Depositor

    SeWon Suh

    Article

    Kang et al Acta Crystallograph Sect F Struct Biol C

    Insert

    PPAT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBS_KS_attB2_SA(2)_T2AGeneSwitch

    Plasmid

    #125216

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBS_KS_attB2_SA(1)_T2AGeneSwitch

    Plasmid

    #125215

    Purpose

    Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS_KS_attB2_SA(0)_T2AGAL4

    Plasmid

    #125211

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBS_KS_attB2_SA(2)_T2AGAL4

    Plasmid

    #125213

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBS_KS_attB2_SA(1)_T2AGAL4

    Plasmid

    #125212

    Purpose

    Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL4
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Thy1.2-NLS_HA_Dre-P2A-BFP

    Plasmid

    #51275

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS_HA_Dre-P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Thy1.2

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. TrmD_HaeIn/pET28b(+) N,C-tag

    Plasmid

    #12665

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28b-AF0623

    Plasmid

    #30164

    Depositor

    SeWon Suh

    Article

    Kim do et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    AF0623 (lig Archaeoglobus fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

44. Thy1.2-Roxed-Cre

    Plasmid

    #51276

    Purpose

    Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Thy1.2

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJK511

    Plasmid

    #71591

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes histidine-222 to glutamine

    Promoter

    T7 promoter

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBS_KS_attB2_SA(2)_T2AGAL80

    Plasmid

    #125219

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL80
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET21a(+)-PA PdxB

    Plasmid

    #12668

    Depositor

    SeWon Suh

    Article

    Ha et al Acta Crystallograph Sect F Struct Biol C

    Insert

    Erythronate-4-phosphate dehydrogenase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. BS.YxiN.Twin1.a207-368.wt

    Plasmid

    #13531

    Depositor

    David McKay

    Article

    Caruthers et al Acta Crystallograph Sect F Struct Biol C

    Insert

    B. subtilis DEAD-box helicase YxiN second domain
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    deleted amino acid residues 1-206 and 369-479

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge