159,557 results

1. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

   Plasmid

   #100146

   Purpose

   Expresses CPT1A

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   CPT1A (CPT1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-TopBP1-mCer

   Plasmid

   #211414

   Purpose

   mammalian expression of human TopBP1 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   TopBP1 (TOPBP1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-puro

   Plasmid

   #45561

   Depositor

   Michael McVoy

   Article

   Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

   Insert

   Puromycin N-acetyl transferase
   

   Use

   Bifunctional selection protein

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Phcmv (Strong human cytomegalovirus immediate ear…

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.4_17G6-1 light chain

   Plasmid

   #198250

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-Ki67-mCherry

   Plasmid

   #183748

   Purpose

   Expresses EGFP-Ki67-mCherry

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Egr1

   Plasmid

   #11729

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   Egr-1 (Egr1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FRE5

   Plasmid

   #62377

   Purpose

   Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   dsRed and GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ALK5 T204D

   Plasmid

   #80877

   Purpose

   mammalian expression of constitutively active ALK5 T204D

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T204D (constitutively active)

   Promoter

   CMV

   Available since

   Aug. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-CMV-3xFLAG-gateway-EGFP

   Plasmid

   #122845

   Purpose

   N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBE45

    Plasmid

    #174655

    Purpose

    CAPTURE method receiver plasmid for Streptomyces heterologous expression

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SIVt

    Plasmid

    #81092

    Purpose

    Single Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2attPC

    Plasmid

    #51547

    Purpose

    DICE donor plasmid without neural transcription factors.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LB601: pMVP/PB/eGFP-DEST

    Plasmid

    #121870

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Use

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3_UnaG-Flag-Sec61B

    Plasmid

    #83413

    Purpose

    Expressed N-term tagged UnaG on Sec61B

    Depositor

    Hyun-Woo Rhee

    Article

    Park et al ACS Chem Biol. 2016 May 27.

    Insert

    UnaG-Sec61B (SEC61B Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBE44

    Plasmid

    #174654

    Purpose

    CAPTURE method 15A origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLL3.7 EGFPC2 TAZ

    Plasmid

    #66850

    Purpose

    Express GFP-fused TAZ by lentivirus

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    WWTR1/TAZ (WWTR1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 35S-GWY-GAL4BD/pAM

    Plasmid

    #201231

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-GAL4BD
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMV306hsp

    Plasmid

    #26155

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP12102 - pAAV-AiE0387m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2102)

    Plasmid

    #208133

    Purpose

    Direct-expressing SYFP2 in astrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST/N1-hBIN1-GFP

    Plasmid

    #27305

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKC96 pBABE H2B-mCherry

    Plasmid

    #192695

    Purpose

    Retroviral transgene expression of human H2B

    Depositor

    Iain Cheeseman

    Article

    Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

    Insert

    H2B
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

    Plasmid

    #208163

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Insert

    iCreR297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SIVh

    Plasmid

    #81091

    Purpose

    Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Ef1a-Con/Fon oG

    Plasmid

    #131778

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    rabies glycoprotein - oG
    

    Use

    AAV; Intrsect

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Use

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-scFvGCN4sfGFPTET1CD

    Plasmid

    #82561

    Purpose

    Expression vector for antibody-sfGFP-TET1CD.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TFORF0589

    Plasmid

    #142653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEJS2424_Nme2.Smu-ABE8e-i8

    Plasmid

    #201522

    Purpose

    Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR4-FLAG (w210-2)

    Plasmid

    #17423

    Purpose

    Gateway entry vector for N-term FLAG tag

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST-PB-R4R3

    Plasmid

    #139518

    Purpose

    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector

    Depositor

    Minoru Ko

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLentiCRISPRv2_mSTING_gRNA_2

    Plasmid

    #196626

    Purpose

    Knock-out STING in murine cells, gRNA 2.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 2 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS42H_PTEF1_dCas9_TCYC1

    Plasmid

    #177155

    Purpose

    Expresses dCas9 in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Insert

    Nuclease-null Cas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p5E-4xnrUAS (L4-R1)

    Plasmid

    #61372

    Purpose

    5' Gateway Entry Clone Containing 4xnrUAS

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnrUAS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge