We narrowed to 8,780 results for: construct
-
Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-L2
Plasmid#141040PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM328
Plasmid#134243PurposeMammalian expression construct expressing sgRNA against PLK1DepositorInsertgRNA targeting PLK1 3'
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45 (delta 154–164)
Plasmid#126879PurposeExpresses crystallisation construct of human Cdc45 in bacteriaDepositorInsertHuman Cdc45 (CDC45 Human)
TagsNo tagExpressionBacterialMutationdeletion amino acid 154-164PromoterT7 promoterAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds
Plasmid#119070PurposeConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled1-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds
Plasmid#119071PurposeConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds
Plasmid#119072PurposeConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled3-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds
Plasmid#119073PurposeConstruct with scrambled-4 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled4-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB03
Plasmid#111961PurposeExpresses MBP-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterTacAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23109Available SinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23117Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9R (XSB525)
Plasmid#78513PurposeConstruct for expressing His10 - SNAPf -9R in E. ColiDepositorInsertSNAPf
TagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9R added to the C-terminal of SNAPfAvailable SinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-Fv2Eflt4um200-2a-tdTomato
Plasmid#67698PurposeExpression constructDepositorAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
ReaLiSM A1-26link-GPIba+2cys
Plasmid#59580Purposeexpress VWF A1-GPIba ReaLiSM construct in mammalian cellsDepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SpELL pBacPAK8
Plasmid#15317DepositorInserteleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
UseBaculovirus constructionTagsFLAGAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SpEAF pBacPAK8
Plasmid#15319DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p073 pCS xGATA4tr + xTRPS1(C119)
Plasmid#11044DepositorUseXenopus, mammalian, avian, and zebrafishMutationFusion construct of XGATA4 (amino acids 1-274) wi…Available SinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
ExpressionBacterialMutationF243I/D238C/S283C/Y127GAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-Bxb1-LP-v2-TC
Plasmid#194327PurposeCLYBL targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable SinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65AAP
Plasmid#92307PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAPDepositorInsertAncestral AAV Capsid Anc80L65AAP
UseAAV and Synthetic BiologyExpressionMammalianPromoterRep2Available SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
Plasmid#222993PurposeMammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianPromoterpCAGAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVPromoterCustomAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
TA1-DuET
Plasmid#213382PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianPromoterEF1aAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR-mKate2
Plasmid#228610PurposeMammalian expression of mKate2, for MuSIC barcode constructionDepositorInsertmKate2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Synthetic, Human)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-RUSH
Plasmid#202799PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1DepositorAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-pgk-hph
Plasmid#233066PurposePiggybac CRISPRa constructDepositorInsertdCas9-VPR
UseCRISPRAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
iCAP-ROOT
Plasmid#192262PurposeAAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsidDepositorInsertSynthetic construct for ROOT VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS
Plasmid#199252PurposeExpression construct encoding the high specificity eSpCas9(1.1)-D10A nickaseDepositorInsertHigh specificity eSpCas9(1.1)-D10A nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW394 (15xUAS::GFP::let-858 3'UTR)
Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
ExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only