We narrowed to 8,877 results for: BLI;
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Plasmid#233926PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutationDepositorInsertCHARMS-3xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-5xA
Plasmid#233928PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutationDepositorInsertCHARMS-5xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-S34Y
Plasmid#233929PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formationDepositorInsertCHARMS-S34Y
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-17xTA-3xAla
Plasmid#233936PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTA with alanine mutation in the TRAF6 binding motifDepositorInsertCHARMS-17xTA-3xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-20xTA-3xAla
Plasmid#233937PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with alanine mutation in the TRAF6 binding motifDepositorInsertCHARMS-20xTA-20xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-TIR-5xT6BMs
Plasmid#233938PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-TIR with 5 TRAF6 binding motifsDepositorInsertCHARMS-TIR-5xT6BMs
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_UENU_donor
Plasmid#221459PurposeVector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.DepositorInsert3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_reporter_vector
Plasmid#221460PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.DepositorInsertgypsy insulator-EGFP-neutral spacer DNA-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE207
Plasmid#120792PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assemblyDepositorInsertInsDown-Mfe
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
B108-Cre2
Plasmid#73990PurposeB108 enhancer of Blimp1 gene drives IRES-Cre expression.DepositorInsertB108-IRES-Cre
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pE-FLP
Plasmid#45978PurposeExpresses yeast Flp driven by the constitutive promoter pE from phage P2DepositorInsertflp
UseSynthetic BiologyTagsExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA
Plasmid#178534PurposeModifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 with human c-terminus, Rat)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
dClover2-C1
Plasmid#54577PurposeLocalization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorTypeEmpty backboneUseTagsdClover2ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMA2640
Plasmid#25434PurposeRetroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selectionDepositorInsertreverse tetracycline-regulated transactivator Advanced
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hUBP43
Plasmid#12455DepositorInsertUBP43 (USP18 Human)
UseTagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EEVEE-CaaX
Plasmid#155225PurposeMPAct with increased linker flexiblityDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE:CloverCP-EFS:ZeoR
Plasmid#68478PurposeLentivirus for establishment of doxycycline-inducible cell line expression CloverCPDepositorInsertsCloverCP
Zeocin Resistance
UseLentiviralTags"CP" degradation domainExpressionMammalianMutationPromoterEFS and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedTagsExpressionMutationPromoterTEF1Available sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEE_GFP
Plasmid#228184PurposeYeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
YEE_RFP
Plasmid#228185PurposeYeast episomal expression vector with type '234' RFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph0
Plasmid#231897PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph1
Plasmid#231898PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph2
Plasmid#231899PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α-loxP-GCaMP6f-loxP-WPRE
Plasmid#233288PurposeAAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.DepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBITE-CD19-CD3
Plasmid#190677PurposeEncodes a modularized form of HD37-OKT3 bispecific antibodyDepositorInsertModularized-HD37-modularized-OKT3-P2A-NeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F263-OKT3
Plasmid#190680PurposeEncodes an EGFRvIII-CD3 BITE protein (F263 clone)DepositorInsertAnti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only