We narrowed to 9,286 results for: Pol
-
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-st1CAS9-pA
Plasmid#131542PurposeExpresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized st1Cas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC167
Plasmid#118909Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C proteaseDepositorInsertNcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-mTq2
Plasmid#67031PurposePlasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2
UseConjugative bacterial vectorAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC193
Plasmid#118903Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
TagsMBP, cleavable with 3C protease; mGFP and TwinStr…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK193
Plasmid#156424PurposeInsect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (Q57H)
TagssfGFP-HisExpressionInsectMutationQ57HPromoterPolyhedrinAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 P13A
Plasmid#12207DepositorInsertPak1 (PAK1 Human)
TagsMycExpressionMammalianMutationP13A: disrupts first polyproline region of Pak1Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOCC93
Plasmid#118911Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagseGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-SmB'
Plasmid#1842DepositorAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5HA2-bglpA
Plasmid#96962PurposeExpression plasmid for HA-tag embedded into human CD52 with short polyADepositorInsertCMV-CD5HA2-bglpA
ExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-CFP-FRBx5
Plasmid#103780Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-CFP-FRBx5
ExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBTK550d
Plasmid#110617PurposeBTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymeraseDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC121
Plasmid#118893Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC70
Plasmid#118878Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, cleavable with TEV protease; HIS6, cleavabl…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS
Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-HcRed
Plasmid#67035PurposePlasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed
UseConjugative bacterial vectorAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJEC626
Plasmid#174367PurposeActivation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.DepositorInsertAlpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
UseSynthetic BiologyExpressionBacterialPromoterJ23106Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
DPAGT1 (DPAGT1A-c005)
Plasmid#98228PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPAGT1A (DPAGT1 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00953:Glycos_transf_4 98-275PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC6
Plasmid#118864Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFPDepositorInsertNcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, EGFPExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagseGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD_OsU3 (GB1184)
Plasmid#68209PurposeProvides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertOsU3 promoter
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC204
Plasmid#118894Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and mKate2, HIS6,…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Plasmid#162918PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase/green fluorescent protein fusion with upstream IRES sequence
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease; GST tagExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK198
Plasmid#156425PurposeInsect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
TagssfGFP-HisExpressionInsectMutationaa 42-275PromoterPolyhedrinAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-mTFP
Plasmid#58327PurposeDestination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genomeDepositorInsertCad-mTFP
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC215
Plasmid#118879Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
TagsDendra2, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-shREV3-4
Plasmid#38033DepositorUseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOCC207
Plasmid#118869Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherryDepositorInsertNcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mCherryExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Nectin-3 in situ probe
Plasmid#45633DepositorInsertNectin-3 in situ probe (Nectin3 Mouse)
UseIn situMutationfragment contains bp# 1626-2039 of NM_021495Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionExpressionInsectPromoterAedes aegypti poly-ubiquitinAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC230
Plasmid#118871Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and mCitrine, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC69
Plasmid#118874Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and mGFP, cleava…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC110
Plasmid#118905Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)DepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and LPETGgExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC219
Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
TagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK L1-L4
Plasmid#62110PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(Q120L)
Plasmid#99701PurposeBacterial expression of RagA-RagC(Q120L)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120LPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C/T180C
Plasmid#66182PurposeExpresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112C, T180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C/L171C
Plasmid#66178PurposeExpresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105C, L171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
D10 pAcGP67a
Plasmid#41812DepositorAvailable SinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP-Prs.AEBP2iso2.mt2K
Plasmid#247010PurposeExpresses human AEBP2iso2.mt2K (with twelve asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertAEBP2iso2.mt2K (AEBP2 Human)
Tags6xHis-MBPExpressionInsectMutationE95K, D96K, E97K, D98K, E99K, E100K, E101K, D102K…PromoterPolyhedrinAvailable SinceNov. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP-Prs.AEBP2iso2.mt2A
Plasmid#247009PurposeExpresses human AEBP2iso2.mt2A (with fifteen asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertAEBP2iso2.mt2A (AEBP2 Human)
Tags6xHis-MBPExpressionInsectMutationE95A, D96A, E97A, D98A, E99A, E100A, E101A, D102A…PromoterPolyhedrinAvailable SinceNov. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD_LcgcC-GFP-H6
Plasmid#245528PurposeHis-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragmentDepositorInsertC-terminal Fragment of the LCGC14 PolB2 Split Intein
TagsHexahistidine Tag and Superfolder Green Fluoresce…ExpressionBacterialAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmRNA-iEMCV-EGFP-A120
Plasmid#225905PurposeIn vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.DepositorInsertsA-inserted T7 class III Φ6.5 promoter
EMCV IRES
EGFP
2x β-globin UTR
poly(A) tail
UseSynthetic Biology; Mrna preparationExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only