4,178 results

1. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

   Plasmid

   #79620

   Purpose

   Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-P25C-GFP

   Plasmid

   #1343

   Depositor

   Li-Huei Tsai

   Insert

   P25 (CDK5R1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4TO-mito-mCherry-10xGCN4_v4

   Plasmid

   #60914

   Purpose

   A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   mito-mCherry
   

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP FAK

   Plasmid

   #50515

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IP GFP-WIPI-1

   Plasmid

   #38272

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEP4 E02S EM2K

   Plasmid

   #20923

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
   

   Use

   Episomal/ebna

   Available Since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

   Plasmid

   #141379

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Flag-HA-USP14

   Plasmid

   #22569

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP14 (USP14 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF.hICN1.CMV.GFP

    Plasmid

    #17623

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-SEP-NRI

    Plasmid

    #23999

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR1 (Grin1 Rat)
    

    Tags

    NR1 signal sequence (aa1-20) and SEP (supereclipt…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTDH3-dCas9-Mxi1

    Plasmid

    #46921

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 Flag MKK7B2Jnk2a2

    Plasmid

    #19727

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Mouse, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSlow-FT-N1

    Plasmid

    #31912

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Expression

    Mammalian

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-RB1-shRNA63

    Plasmid

    #25641

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 63 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. MG15-luc (mut p53 binding sites)

    Plasmid

    #16443

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    p53-binding site (mutant) (TP53 )
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-Beclin1 (FL)

    Plasmid

    #24388

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 Flag MKK7B2Jnk1a1(APF)

    Plasmid

    #19730

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(1959)-Pro-…

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

    Plasmid

    #141367

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge