We narrowed to 6,451 results for: siae
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Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKP112 (pRS406-GFP-ADH1t)
Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneTagsGFPExpressionBacterial and YeastPromoterNoneAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS240
Plasmid#34966PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationK533∆, high-affinity epitopePromoterGAL1Available SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
Promotertight TREAvailable SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)
Plasmid#86417Purposeluminal split GFP11 reporter with mCherryDepositorInsertNOP1pr-mCherry-SCS2TM-GFP11
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2::p65Uw
Plasmid#26229DepositorInsertGAL4::p65 (RELA Human, Budding Yeast)
ExpressionInsectAvailable SinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2795
Plasmid#63650PurposepCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)
Plasmid#86414Purposenuclear split GFP11 reporter without mCherryDepositorInsertNOP1pr-GFP11-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1prAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV126
Plasmid#63192PurposepRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
TagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
TagsmScarlet fused to FKBPExpressionYeastPromoterSP6Available SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHES830
Plasmid#87944PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
ExpressionYeastPromoterADH1 (crippled)Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
Tags3xHAExpressionYeastMutation∆RPromoterpATG8Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407A, I532AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFvl160
Plasmid#64753PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gal4DB-NS3-VP64-p65_IRES_mCherry
Plasmid#112627PurposeEncodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.DepositorInsertGal4DB-NS3-VP64-p65
TagsAU1 (N term on NS3), HA (C term on NS3), and T7 (…ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP5052
Plasmid#128729PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAM1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AKARdual
Plasmid#99869PurposeMeasure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertAKAR4
TagsLSSmOrange and mKate2ExpressionMammalianPromoterCMVAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPEnvy-SpHis5
Plasmid#60782PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPEnvy using SpHis5 selectionDepositorInsertGFPEnvy
UseYeast integratingMutationS30R Y39N T65C S72A F99S N105T Y145F M153T V163A …Available SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP3915
Plasmid#113335PurposeBeta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertArtificial Transcription Factor (McIsaac et al, 2013)
ExpressionYeastAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV116
Plasmid#63183PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only