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Showing: 6841 - 6880 of 10097 results

  5. pGBW-m4134547

    Plasmid
    #152583
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152583-plasmid-map-sequence-id-306155

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  6. pGBW-m4133502

    Plasmid
    #153565
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153565-plasmid-map-sequence-id-300141

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  7. pGBW-m4133717

    Plasmid
    #152919
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152919-plasmid-map-sequence-id-317263

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  8. pGBW-m4134336

    Plasmid
    #152014
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152014-plasmid-map-sequence-id-300229

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  12. mCherry-FDD

    Plasmid
    #80629
    Purpose
    Fluorescent destablizing domain for regulating protein stability and fluorescence
    Depositor
    Thomas Wandless
    Article
    Navarro et al ACS Chem Biol. 2016 Jun 6.
    Insert
    UnaG
    Use
    Retroviral
    Tags
    HA-mcherry-linker
    Expression
    Mutation
    mutations A36V, R136G
    Promoter
    LTR
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    80629-plasmid-map-sequence-id-156876

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  15. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  16. pFRT-TODestGFP_HuD

    Plasmid
    #65763
    Purpose
    expresses GFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65763-plasmid-map-sequence-id-115023

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  17. pGBW-m4133361

    Plasmid
    #154789
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154789-plasmid-map-sequence-id-307385

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  19. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  20. pE2c

    Plasmid
    #17462
    Purpose
    modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    3xHA
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17462-plasmid-map-sequence-id-140110

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  21. pBabe puro IkB alpha M

    Plasmid
    #12332
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  22. pFRT-TODestFLAGHA_HuC

    Plasmid
    #65756
    Purpose
    expresses FLAGHA-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65756-plasmid-map-sequence-id-111826

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  23. pFRT-TODestGFP_HuC

    Plasmid
    #65762
    Purpose
    expresses GFP-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65762-plasmid-map-sequence-id-111834

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  24. pcDNA_DiCre2_60.F2

    Plasmid
    #60210
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 60-343 …
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60210-plasmid-map-sequence-id-96296

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  25. pE2n

    Plasmid
    #17456
    Purpose
    modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    3xHA
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17456-plasmid-map-sequence-id-8624

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  26. pFRT-TODestFLAGHA_HuD

    Plasmid
    #65757
    Purpose
    expresses FLAGHA-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65757-plasmid-map-sequence-id-111829

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  32. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  36. pJYDC2

    Plasmid
    #162459
    Purpose
    Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162459-plasmid-map-sequence-id-321477

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  37. pFRT-TODestFLAGHA_HuB

    Plasmid
    #65755
    Purpose
    expresses FLAGHA-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    65755-plasmid-map-sequence-id-121638

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  38. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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Showing: 6841 - 6880 of 10097 results