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Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorInsertH2B targeting sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA1
Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJZ002
Plasmid#120231PurposeCRISPR-cas9 targeting in E. coli with ampicillin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA-1050S (UASt-dCasRx)
Plasmid#132419PurposeExpress dCasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
UseTagsExpressionInsectMutationPromoterUAStAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6
Plasmid#159913PurposeMutagenesis of Kcnj6DepositorInsertKcnj6 (Kcnj6 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (wtAcrIIA4)
Plasmid#128115PurposeExpresses SpyCas9 fused to wild-type AcrIIA4DepositorInsertCascAID (wtAcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF204
Plasmid#121656PurposeU6-sgRNA EFS-Cas9-wt-P2A-PuroDepositorInsertCas9
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
S12_EcoRV_PGK_AscI
Plasmid#128476PurposePGK promoterDepositorInsertPGK promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3.1.0-gDNA
Plasmid#112396PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DDIT3DepositorInsertDDIT3 (DDIT3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF503
Plasmid#135735PurposeDonor vector for 3' FLAG tag of human ZNF503DepositorInsertZNF503 Homology arms (ZNF503 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC2102-reci
Plasmid#117171PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLSDepositorInsertreci_wtCas9 without NLS
UseLentiviralTagsExpressionMutationPromoterU6 PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-NT
Plasmid#164861PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-NT
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-1
Plasmid#164858PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-1
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_Psyn-sgRNA500
Plasmid#149557PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsyn and pSynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH184
Plasmid#122857PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCREB1.1.0-gDNA
Plasmid#113770PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CREB1DepositorInsertCREB1 (CREB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDC2.1.0-gDNA
Plasmid#113781PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2DepositorInsertCSDC2 (CSDC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_4
Plasmid#79863PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF331
Plasmid#135726PurposeDonor vector for 3' FLAG tag of human ZNF331DepositorInsertZNF331 Homology arms (ZNF331 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMST665_BB3_L 23_gRNAempty_cas9_BbsI
Plasmid#90278PurposeBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9DepositorInsertCas9
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_1
Plasmid#79860PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_2
Plasmid#79861PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Drosha_3
Plasmid#79862PurposeCRISPR/Cas9 DROSHADepositorInsertsgRNA Drosha
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF226
Plasmid#121667PurposeEFS-Cas9-wt-P2A-PuroDepositorInsertcas9
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAEF6
Plasmid#136303PurposepAEF5 plasmid with two gRNA insertedDepositorInsertdouble cassette coding for gRNA
UseCRISPR; Template for pcr to insert double grna co…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY103
Plasmid#130937PurposeEngineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK.1.0-gDNA
Plasmid#112406PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CLOCKDepositorInsertCLOCK (CLOCK Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF580
Plasmid#86278PurposeDonor vector for 3' FLAG tag of human ZNF580DepositorInsertZNF580 homology arms (ZNF580 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SAP130
Plasmid#86267PurposeDonor vector for 3' FLAG tag of human SAP130DepositorInsertSAP130 homology arms (SAP130 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOXO4.1.0-gDNA
Plasmid#112408PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXO4DepositorInsertFOXO4 (FOXO4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 125-190
Plasmid#99664PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain ( 125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC35
Plasmid#62325PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL31-sgRNANT1-KmR
Plasmid#73221PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL31-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL31Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC102
Plasmid#62337PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEL846
Plasmid#214236PurposeFrCas9 genome editing plasmid in riceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEL847
Plasmid#214237PurposeTREX2-FrCas9 genome editing in riceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEL848
Plasmid#214238PurposeFrCas9 cytosine base editing plasmid V2.1 in riceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEL849
Plasmid#214239PurposeFrCas9 adenine base editing plasmid in riceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
LsgRNA-MS2
Plasmid#235597PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAT22
Plasmid#204311PurposeFor generating targeting inversions. N-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPn
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1p6_barley_sgRNA_scaffold
Plasmid#231033PurposeCloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6DepositorInsertScaffold for Cas9 mediated CRISPR
UseCRISPRTagsExpressionBacterialMutationPromoterTaU6Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1p5_barley_sgRNA_scaffold
Plasmid#231032PurposeCloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5DepositorInsertScaffold for Cas9 mediated CRISPR
UseCRISPRTagsExpressionBacterialMutationPromoterTaU6Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1p4_barley_sgRNA_scaffold
Plasmid#231031PurposeCloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4DepositorInsertScaffold for Cas9 mediated CRISPR
UseCRISPRTagsExpressionBacterialMutationPromoterTaU6Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1p3_barley_sgRNA_scaffold
Plasmid#231030PurposeCloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3DepositorInsertScaffold for Cas9 mediated CRISPR
UseCRISPRTagsExpressionBacterialMutationPromoterTaU6Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-3P5P
Plasmid#219680PurposeCROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNAExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-mir1-8mer4x (pEZ184)
Plasmid#229058PurposeDual-luciferase reporter containing 4 copies of the 8mer site of human miR-1DepositorInsertHuman miR-1 binding sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRC065d
Plasmid#225979PurposedRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)DepositorInsertsdRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS
UseCRISPRTagsExpressionMutationPromoterJ23107 and J23119Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only