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We narrowed to 18,393 results for: Tec

Showing: 8551 - 8600 of 18393 results

  3. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183765-plasmid-map-sequence-id-361682

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  4. pN094

    Plasmid
    #167155
    Purpose
    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-npgA-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167155-plasmid-map-sequence-id-335276

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  5. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72250-plasmid-map-sequence-id-131871

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  7. pGLO-GFP-3UAG

    Plasmid
    #82501
    Purpose
    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Green Fluorescence Protein with 3 UAG codons
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Added an UAG codon at the amino acid position 3, …
    Promoter
    AraBAD
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    82501-plasmid-map-sequence-id-156568

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  10. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid
    #131313
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    131313-plasmid-map-sequence-id-254655

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  11. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

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  12. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  19. MSCV-opEBNA2-IRES-mCherry

    Plasmid
    #220355
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220355-plasmid-map-sequence-id-437048

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  28. pHRIG-PRAS40

    Plasmid
    #53599
    Purpose
    lentiviral expression of constitutively active PRAS40 and EGFP
    Depositor
    Heng Zhao
    Article
    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1
    Insert
    PRAS40 (AKT1S1 Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and myc
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pET19-MTdTc302

    Plasmid
    #207458
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc302 (Dntt Mouse)
    Use
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207458-plasmid-map-sequence-id-407772

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  50. pSHUT4-eYFP

    Plasmid
    #133094
    Purpose
    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI
    Depositor
    Jens Laurids Sørensen
    Article
    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25
    Insert
    enhanced yellow flourescent protein
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    TEF-1α
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133094-plasmid-map-sequence-id-266874

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Showing: 8551 - 8600 of 18393 results