161,641 results

1. pET28a-Ec.coaBC (pCLK1210)

   Plasmid

   #50448

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

   Insert

   PPCS PPCDC
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEHIShqprt

   Plasmid

   #92166

   Purpose

   Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

   Depositor

   James Naismith, Huanting Liu

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   Quinolinate Phosphoribosyltransferase (QPRT Human)
   

   Use

   Tags

   TEV protease -cleavable N-terminal 6His

   Expression

   Bacterial

   Mutation

   changed Asp 2 to Asn to create BspHI site

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBABE.puro/TO/Flag/tGFP-HuR.wt

   Plasmid

   #110349

   Purpose

   Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   gag

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dTomato_LD 0_CS M_tTA-BFP

   Plasmid

   #58875

   Purpose

   MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 891 pFastBac GST PTEN

   Plasmid

   #10937

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5/FRT-HA-hM3D(Gq)

   Plasmid

   #45547

   Purpose

   Expresses hM3D (Gq) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   hM3D (Gq)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB2195

   Plasmid

   #67548

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. HRE-dUnOHR

   Plasmid

   #201714

   Purpose

   Hypoxia responsive promoter driving UnaG and mOrange

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4.26-SS-79

   Plasmid

   #68740

   Purpose

   ZF2 Op -- GFP

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   ZF2 Op
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJC-20xUAS-TALE3-VP64-P10

    Plasmid

    #104607

    Purpose

    Expresses TALE3 under UAS control

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    TALE3
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJC-20xUAS-TALE4-VP64-P10

    Plasmid

    #104608

    Purpose

    Expresses TALE4 under UAS control

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    TALE4
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag

    Plasmid

    #236861

    Purpose

    Express NanoLuc(R)-HRAS WT-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    HRAS (HRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Tad-CBE(TCG2)

    Plasmid

    #235068

    Purpose

    Expresses Tad-CBE(TCG2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCG2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tad-CBE(CCA3)

    Plasmid

    #235070

    Purpose

    Expresses Tad-CBE(CCA3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCA3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tad-CBE(CCT2)

    Plasmid

    #235071

    Purpose

    Expresses Tad-CBE(CCT2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCT2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tad-CBE(TCC2)

    Plasmid

    #235067

    Purpose

    Expresses Tad-CBE(TCC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMTKM05

    Plasmid

    #233477

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-DIO-Bmal1

    Plasmid

    #239045

    Purpose

    Overexpression of Bmal1

    Depositor

    Qingchun Tong

    Article

    Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

    Insert

    mArntl (Bmal1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Bmal1

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP13899 - pAAV-AiE0494m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3899)

    Plasmid

    #230385

    Purpose

    AiE0494m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP1365 - pAAV-AiE2136m-minBG-SYFP2-WPRE3-BGHpA

    Plasmid

    #230630

    Purpose

    AiE2136m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AiP1376 - pAAV-AiE2147m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #220645

    Purpose

    AiE2147m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP11576 - pAAV-hsA2-AiE0082m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1576)

    Plasmid

    #230354

    Purpose

    AiE0082m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minRho

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AiP12861 - pAAV-AiE0666m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2861)

    Plasmid

    #230139

    Purpose

    AiE0666m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1/V5-His-mPer1

    Plasmid

    #219421

    Purpose

    Expresses 3873 bp of mPer1 coding sequence in E. coli.

    Depositor

    Aziz Sancar

    Article

    Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mPer1 (Per1 Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP1962 - pAAV-AiE2591m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214592

    Purpose

    AiE2591m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiP1425 - pAAV-AiE2132m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214495

    Purpose

    AiE2132m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UMG-SP-3_pET29b

    Plasmid

    #215435

    Purpose

    Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NfPolyA_pET21b

    Plasmid

    #215413

    Purpose

    Expression construct for NfPolyA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NfPolyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NPY1R-DuET

    Plasmid

    #213354

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPY1R (NPY1R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge