159,461 results

1. Talin-CS

   Plasmid

   #83377

   Purpose

   Control FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted at C-terminal o…

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131A

   Plasmid

   #69273

   Purpose

   Golden Gate entry vector; 1st gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRK5-FLAG-Bag6

   Plasmid

   #61836

   Purpose

   express N-terminally FLAG tagged human Bag6

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   Bag6 (BAG6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCLXSN(GFP)-mTRAF2

   Plasmid

   #174736

   Purpose

   Gene overexpression

   Depositor

   Shao-Cong Sun

   Insert

   TRAF2 (Traf2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLC-Flag-CRBN-P2A-Hygro

   Plasmid

   #124303

   Purpose

   Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CRBN (CRBN Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-mCherry FP

   Plasmid

   #52114

   Purpose

   a bicistronic IRES-mCherry containing retroviral vector

   Depositor

   Dario Vignali

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-mDyn3 pcDNA3

   Plasmid

   #36265

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 3 (Dnm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

8. MIGR-RORgt

   Plasmid

   #24069

   Depositor

   Dan Littman

   Insert

   Rorgt (Rorc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-FR-GECO1c

   Plasmid

   #163682

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1c

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

    Plasmid

    #182251

    Purpose

    Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-V0A

    Plasmid

    #210347

    Purpose

    Expresses ATP6V0A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0A (ATP6V0A1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p36-p37-p38-p40-pET-Duet-1

    Plasmid

    #175049

    Purpose

    Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ132A

    Plasmid

    #69274

    Purpose

    Golden Gate entry vector; 2nd gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Scarlet-Kif5a

    Plasmid

    #118745

    Purpose

    Expresses scarlet tagged Kif5a

    Depositor

    Beverly Koller

    Insert

    Kif5a cDNA (Kif5a Mouse)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4.TO-K5-2xCSTREP

    Plasmid

    #136154

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-STAT3-S727D

    Plasmid

    #73364

    Purpose

    Expression of STAT3-S727D

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Use

    Tags

    FLAG tagged

    Expression

    Mammalian

    Mutation

    E16K S727D

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

    Plasmid

    #196219

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG FLEX iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

21. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Strep-KIFC1*-mCh

    Plasmid

    #120169

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUb FLAG-Human IntS6

    Plasmid

    #198408

    Purpose

    Expresses FLAG-tagged human IntS6

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    DDX26 (INTS6 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMito-LSSmOrange

    Plasmid

    #37135

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ube2v1

    Plasmid

    #31429

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2V1 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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