165,904 results

1. pRS314-T7-GFP

   Plasmid

   #33130

   Depositor

   Michael Rosbash

   Article

   Dower et al RNA. 2002 May;8(5):686-97.

   Insert

   GFP with TDH3 5' and 3' UTR (gfp )
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3109 (pSpy1C-Plac(1)_ffluc)

   Plasmid

   #218514

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(1)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-NotIR (GC)

   Plasmid

   #165507

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   NotI (GC-rich recognition site)
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-CMV-GFP-W

   Plasmid

   #196046

   Purpose

   lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Promoter

   CMV

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM106B-EGFP

   Plasmid

   #210366

   Purpose

   Expresses TMEM106B fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   TMEM106B (TMEM106B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

   Plasmid

   #236368

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   D156R, D235R, E292R, D350R

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. hGFAP-GFP

   Plasmid

   #40592

   Depositor

   Albee Messing

   Article

   Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

   Insert

   GFP-S65T
   

   Use

   Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Bacterial and Mammalian

   Mutation

   Construct contains a mutated, humanized hGFP(S65T…

   Promoter

   hGFAP promoter

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cK_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104910

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito-mStayGold_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221898

    Purpose

    Expresses mStayGold targeted to the mitochondria

    Depositor

    Janelia Integrative Imaging

    Insert

    cox8-mStayGold
    

    Tags

    Cox8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

    Plasmid

    #178960

    Purpose

    Expresses saCas9 protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Staphylococcus aureus Cas9 (SaCas9)
    

    Use

    AAV

    Tags

    3x HA

    Promoter

    gfaABC1D (astrocyte specific)

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. NES-Grx1-roGFP2

    Plasmid

    #236551

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    NES-Grx1-roGFP2 (NES Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-CMV-tagRFP-FLAG-cGAS

    Plasmid

    #86676

    Purpose

    Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    tagRFP-FLAG-cGAS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUNIV backbone

    Plasmid

    #24705

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179120

    Purpose

    Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    control sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GPRC6A-Tango

    Plasmid

    #66386

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLSL.R.Ly

    Plasmid

    #227697

    Purpose

    Reengineered geminiviral replicon vector

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-hnRNPU-V5

    Plasmid

    #35974

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

    Plasmid

    #201684

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pp-mCAT1

    Plasmid

    #83948

    Purpose

    This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

    Depositor

    Vincent Lotteau, Philippe Mangeot

    Article

    Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

    Insert

    mCAT1 (Slc7a1 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. AKT-translocation-mStrawberry reporter

    Plasmid

    #158685

    Purpose

    This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Expression

    Bacterial

    Promoter

    PA1

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. c-myc-PT3EF1a

    Plasmid

    #92046

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Xin Chen

    Article

    Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

    Insert

    C-MYC (MYC Human)
    

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    July 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBABE-puro-GFP-wt-lamin A

    Plasmid

    #17662

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

    Insert

    GFP-lamin A (LMNA Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGAMA-empty

    Plasmid

    #74755

    Purpose

    Mammalian lentiviral vector

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1 Dendra2

    Plasmid

    #103967

    Purpose

    Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    Dendra2
    

    Expression

    Mammalian

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEGFP-puro

    Plasmid

    #45561

    Depositor

    Michael McVoy

    Article

    Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

    Insert

    Puromycin N-acetyl transferase
    

    Use

    Bifunctional selection protein

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Phcmv (Strong human cytomegalovirus immediate ear…

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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