We narrowed to 14,039 results for: TIM

1. pMIII-Neo-BFP

   Plasmid

   #153426

   Purpose

   Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   mTagBFP2
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mt deltaN20 TFB2MdeltaC3

   Plasmid

   #205062

   Purpose

   TFB2M protein expression

   Depositor

   Smita Patel

   Article

   Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

   Insert

   TFB2M (TFB2M Human)
   

   Expression

   Bacterial

   Mutation

   Lacks N-terminal 20 aa and C-terminal 3 aa

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ptagRFP-MS2coatProtein

   Plasmid

   #103831

   Purpose

   Live cell reader for MS2 loop RNA production

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   MS2 coat protein (MCP) (cp )
   

   Tags

   tagRFP

   Expression

   Mammalian

   Mutation

   MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Plasmid

   #163073

   Purpose

   Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Grx1
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCG-TPMV Hd32-IL-13

   Plasmid

   #163869

   Purpose

   Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV H glycoprotein/IL-13
   

   Tags

   Human IL-13

   Expression

   Mammalian

   Mutation

   Amino acids 2 to 33 at N terminus deleted

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

   Plasmid

   #63169

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-FLAG"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

    Plasmid

    #191457

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROF417

    Plasmid

    #155335

    Purpose

    Constitutive expression LFY-VP16-2A-RLuc in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SARS-CoV-2-E

    Plasmid

    #215814

    Purpose

    Expresses SARS-CoV-2 Envelope protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Envelope (E )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SARS-CoV-2-M

    Plasmid

    #215813

    Purpose

    Expresses SARS-CoV-2 Membrane protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Membrane (M )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-SARS-CoV-2-S (D614G)

    Plasmid

    #215838

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Promoter

    Ptet

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Twitch-4 pcDNA3

    Plasmid

    #49533

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-4
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pJT9R

    Plasmid

    #98305

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMY-empty-MCS

    Plasmid

    #163351

    Purpose

    The empty pMY backbone with multiple cloning site

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2/pr-Membrane

    Plasmid

    #120817

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    pr-Membrane ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol2pA2-mnx1:MLS-KillerRed

    Plasmid

    #115515

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Promoter

    -3mnx1

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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