We narrowed to 17,706 results for: jun

1. pAP24

   Plasmid

   #70190

   Purpose

   Modular vector for secreted luciferase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   sLucopt
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rtn2b-mNeon

   Plasmid

   #186598

   Purpose

   Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B (RTN2 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RNF168-Halo HRD

   Plasmid

   #207080

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Expression

   Mammalian

   Promoter

   None

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVG122

   Plasmid

   #211765

   Purpose

   Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   strongLOV
   

   Use

   For single-copy integration (if cut with pmei)

   Tags

   SV40 NLS and VP16

   Expression

   Yeast

   Mutation

   compared to EL222, strongLOV contains Glu84Asp mu…

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Jnk3a1

   Plasmid

   #29577

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Jnk3a1 (MAPK10 Human)
   

   Tags

   HIS6 and TEV

   Expression

   Bacterial

   Mutation

   Contains residues 40-402

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. MKK2

   Plasmid

   #29580

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   MKK2 (MAP2K2 Human)
   

   Tags

   GST, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   Constitutively active mutant; Starting Met remove…

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pMJ106: pRS426-His-MBP-NlovFz2_Native

   Plasmid

   #205253

   Purpose

   S. cerevisiae expression vector for NlovFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   NlovFz2
   

   Tags

   10xHis, MBP

   Expression

   Yeast

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMJ45: pRS426-His-MBP-SpuFz1_Native

    Plasmid

    #205252

    Purpose

    S. cerevisiae expression vector for SpuFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-NLS-ZapCV2-ALA

    Plasmid

    #203665

    Purpose

    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTet-tns

    Plasmid

    #170628

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTnsABC
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-Rsn2

    Plasmid

    #61686

    Purpose

    bacterial expression of ranaspumin 2 as a His-tagged fusion protein

    Depositor

    Brian Smith

    Article

    Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

    Insert

    Ranaspumin-2
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cr3Ec

    Plasmid

    #184860

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 RagB shRNA #1

    Plasmid

    #26627

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagB shRNA (RRAGB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    10xHis, MBP

    Expression

    Yeast

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2XMyc-LRRK2-K1347A

    Plasmid

    #25365

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Lysine 1347 mutated to Alanine

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pMJ332: pU6-GtFz1_omegaRNA-entry

    Plasmid

    #205265

    Purpose

    Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRetroSuper-GFP Smad3

    Plasmid

    #15723

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    Smad3 shRNA (SMAD3 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

20. POLN_pDEST17

    Plasmid

    #64885

    Purpose

    bacterial expression of POLN for purification

    Depositor

    Richard Wood

    Article

    Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

    Insert

    POLN (POLN Human)
    

    Tags

    Flag and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge