19,635 results

1. mCherry-Caveolin-C-10

   Plasmid

   #55008

   Purpose

   Localization: Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

   Insert

   Caveolin (CAV1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. tFucci(SA)5

   Plasmid

   #153520

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(SA)5.
   

   Tags

   AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.2-ccdB-3xFLAG-V5

   Plasmid

   #87071

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA6A-EGFR WT

   Plasmid

   #42665

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR (EGFR Human)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cbh_v5 AAV-ABE C-terminal

   Plasmid

   #137178

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVXT Sox4

   Plasmid

   #101121

   Purpose

   Sox4 coding region

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SOX4 (Sox4 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-FLAG-SZT2

   Plasmid

   #87034

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV MCS mCherry-KASH

    Plasmid

    #139654

    Purpose

    Cloning template for AAV-based ORANGE knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MED17

    Plasmid

    #15410

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

    Plasmid

    #192616

    Purpose

    AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    FLEX-RIboL1-jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV.GFAP.iGABASnFR

    Plasmid

    #112172

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR
    

    Use

    AAV

    Promoter

    GFAP

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF CAG luc hygro

    Plasmid

    #67502

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-Mfn2

    Plasmid

    #141156

    Purpose

    Mammalian expression and labeling mitofusin 2

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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