20,093 results

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSynSRE-T-Luc

Plasmid

#60444

Purpose

Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

Depositor

Timothy Osborne

Article

Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

Insert

HMG-CoA synthase promoter (-324/-225)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-Caveolin-C-10

Plasmid

#55008

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-TOMM20-N-10

Plasmid

#54282

Purpose

Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-HDAC4

Plasmid

#45636

Depositor

Tso-Pang Yao

Article

Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

Insert

HDAC4 (HDAC4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt3A-V5

Plasmid

#43810

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT3A (WNT3A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pBMPC3

Plasmid

#31367

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Bmal1 (Bmal1 Mouse)

Tags

His

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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