We narrowed to 5,783 results for: RIK

1. H513 px330.sgActin.UTR.2

   Plasmid

   #138176

   Purpose

   CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

   Depositor

   Wen Xue

   Article

   Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

   Insert

   sgActin (Actb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EGFP.T2A.NCLX

   Plasmid

   #181873

   Purpose

   Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Inserts

   solute carrier family 8 member B1 (Slc8a1 Mouse)
   EGFP
   

   Use

   AAV and Adenoviral

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   synthetic hybrid CAG promoter and synthetic hybri…

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

   Plasmid

   #174634

   Purpose

   Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   mCherry-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-f1(+)-ACTB-mEGFP

   Plasmid

   #115909

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP (ACTB Human)
   

   Use

   Donor template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Human, Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Tbg-CES2A-ΔC-S227A

   Plasmid

   #200560

   Purpose

   Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   CMV promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetO-FUW-Mecom 2

   Plasmid

   #118591

   Purpose

   Expresses Mecom isoform 2 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Mecom (Mecom Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNL3.1-Erg

   Plasmid

   #182706

   Purpose

   Express Erg enhancer in mammalian cells

   Depositor

   Robert Rowe

   Article

   Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

   Insert

   Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

    Plasmid

    #199262

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

    Plasmid

    #199265

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16-mHP1alpha

    Plasmid

    #181910

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. nAChR beta2 CFP

    Plasmid

    #15106

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-CFP-M (Chrnb2 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

15. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-sACE2-WT(732)

    Plasmid

    #154098

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NCKX4 scFv [N414/25]

    Plasmid

    #190553

    Purpose

    Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

    Plasmid

    #74919

    Purpose

    Mammalian expression of mKlf2 and mNanog-mCherry

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Inserts

    Klf2 (Klf2 Mouse)
    Nanog (Nanog Mouse)
    

    Use

    Tags

    T2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

    Plasmid

    #174134

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Only contains the sequences coding for amino acid…

    Promoter

    hSyn

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. attB-GFP-Tet1-Poly(A)

    Plasmid

    #65559

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2-HA-Mettl3

    Plasmid

    #196262

    Purpose

    In vitro transcription/translation of HA-tagged wild-type Mettl3

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCEP4-FLAG-CXCR4-VN

    Plasmid

    #98964

    Purpose

    FLAG-tagged human CXCR4 fused to N-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    FLAG (dykdddd) epitope tag, Signal/leader sequenc…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Synaptotagmin-6 scFv [N270/47]

    Plasmid

    #190544

    Purpose

    Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCEP4-myc-CXCR4-VC

    Plasmid

    #98967

    Purpose

    myc-tagged human CXCR4 fused to C-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid

    #199263

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

    Plasmid

    #105024

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

    Plasmid

    #99261

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    Y705F (contains a silent mutation: nucleotide 271…

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sgCRY2

    Plasmid

    #197457

    Purpose

    Expresses sgRNA(CRY2 gene) in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
    

    Use

    AAV

    Tags

    mCherry (K14E, N210D, E249G)

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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