We narrowed to 18,567 results for: puro

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pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

Plasmid

#120564

Purpose

Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Publication

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Publication

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Publication

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-RAB10

Plasmid

#130883

Purpose

Expresses human RAB10 with N-terminal GFP tag

Depositor

Michael Bassik

Publication

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLCKO2

Plasmid

#125518

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

Depositor

Jason Moffat

Publication

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Publication

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Tags

Flag

Expression

Mammalian

Mutation

*

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRAF6mut-UTR

Plasmid

#15094

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMSCV mLST8 myc (aka: GbL-myc)

Plasmid

#12580

Depositor

David Sabatini

Publication

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mLST8 (MLST8 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

Plasmid

#221234

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Use

Lentiviral

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-HA-PuroR

Plasmid

#80959

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA tag

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_TagBFP

Plasmid

#113724

Purpose

Expresses the fluorescent protein TagBFP in a lentiviral backbone

Depositor

Vanessa LaPointe

Insert

TagBFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PresentER-Cassette GFP

Plasmid

#102942

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

Depositor

David Scheinberg

Publication

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLIX402 PHF2

Plasmid

#91700

Purpose

inducible lentiviral expression of PHF2

Depositor

Bob Weinberg

Publication

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SP6-VEE-GFP

Plasmid

#58976

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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