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pWZL Neo Myr Flag AXL

Plasmid

#20428

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AXL (AXL Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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VHL-pGex2TK

Plasmid

#20790

Depositor

William Kaelin

Publication

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

VHL (VHL Human)

Tags

GST

Expression

Bacterial

Mutation

VHL wt cDNA, no mutations

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFRT/FLAG/HA-DEST EIF2C2

Plasmid

#19888

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA5/FRT/TO HIS HSPA8

Plasmid

#19541

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

His

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRK5-HA GST RagD wt

Plasmid

#19307

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Publication

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

aa2-439

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pFA6a-5FLAG-KanMX6

Plasmid

#15983

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Type

Empty backbone

Tags

5xFlag

Expression

Bacterial

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA 3.1(-) mouse C/EBP beta (LIP)

Plasmid

#12561

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)

Expression

Mammalian

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pLIA mNIC myc (CC#341)

Plasmid

#15131

Depositor

Connie Cepko

Publication

Bao et al J Neurosci. 1997 Feb 15. 17(4):1425-34.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753.…

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pQLinkG2

Plasmid

#13671

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

GST, TEV, and attB1

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pARF1-CFP

Plasmid

#11381

Depositor

Joel Swanson

Publication

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Publication

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pcDNA3-dnCDK5

Plasmid

#1344

Depositor

Li-Huei Tsai

Insert

dnCDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Mutation

D144N

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pTRE-GFP-NMHC II-A

Plasmid

#10844

Depositor

Robert Adelstein

Publication

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Pa01 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Publication

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

Plasmid

#185912

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpRY-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CMV and T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CoV2-N-WT-Hu1

Plasmid

#177937

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )

Expression

Mammalian

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3.3_CoV2_B.1.1.7

Plasmid

#170451

Purpose

Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

Depositor

David Nemazee

Publication

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))

Tags

NA

Expression

Mammalian

Mutation

HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Publication

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HyPer7.2DAAO-NES

Plasmid

#168301

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

Depositor

Thomas Michel

Publication

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NES

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Publication

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP13

Plasmid

#141266

Purpose

Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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