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Addgene

We narrowed to 10,694 results for: AGA

Showing: 5251 - 5280 of 10694 results
  1. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

    Plasmid
    #149278
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Insert
    Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Promoter
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pLT 652

    Plasmid
    #59997
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Inserts
    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. STARR-seq luciferase validation vector_SCP1_SV40

    Plasmid
    #99311
    Purpose
    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    SV40 enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pLCHKO_GFP_Luciferase

    Plasmid
    #155079
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Insert
    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. p426_Cas9_gRNA-ARS308a

    Plasmid
    #87384
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. STARR-seq luciferase INF enhancer vector_ORI_IFI27

    Plasmid
    #99320
    Purpose
    Luciferase validation vector with IFI27 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    enhancer; chr14: 94575894-94578286
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 5251 - 5280 of 10694 results