12,913 results

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pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pMX-mPGK-CD90.2-Rluc_miR

Plasmid

#163332

Purpose

Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mPGK

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ly6G-miR30

Plasmid

#163358

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pHXPL

Plasmid

#17667

Depositor

Cecilia Lo

Insert

Cx43 promoter (Gja1 Mouse)

Tags

LacZ

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a-TAF4(827-909)

Plasmid

#105620

Purpose

bacterially express TAF4 HFD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4-HFD(827-909) (Taf4 Mouse)

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-TAF12(50-130)

Plasmid

#105618

Purpose

bacterially express GST tagged TAF12 HFD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12-HFD amino acid 50-130 (Taf12 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
Tmem98-mTq2

Plasmid

#124504

Purpose

Expresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Promoter

U6

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
3389 pcDNA3 Bid p22

Plasmid

#8807

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p22 (Bid Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
Gal4 PGC1 alpha L2/3A

Plasmid

#8898

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

PGC-1a (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Mutation

LXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ELK1

Plasmid

#98616

Purpose

Gateway entry TOPO TA cloning vector for ELK1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 (Elk1 Mouse)

Use

Topo ta cloning vector

Mutation

Q230R

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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UR1Ct (265-1040)

Plasmid

#115647

Purpose

expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag1 (265-1040) (Rag1 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag1 (aa 265-1040)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A/669A

Plasmid

#19283

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
XE212 RGS9-pcDNA3.1

Plasmid

#16676

Depositor

Randall Moon

Insert

RGS9 (Rgs9 Mouse)

Expression

Mammalian

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx3

Plasmid

#74342

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 3 (Dlx3 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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BD46.32

Plasmid

#45559

Depositor

Gregory Pazour

Article

Pazour et al Curr Top Dev Biol. 2008;85:115-49. doi:

Insert

Sstr3 (Sstr3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
p5xFbx15 (-544/-527)-TK-Luc/Sox-m1

Plasmid

#15687

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x mutated Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Mutation

Mutated in Sox binding site

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse eIF2C4

Plasmid

#50356

Purpose

Mouse eIF2C4 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C4 (Ago4 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-Flag-Snrnp40

Plasmid

#134253

Purpose

Lentivector encoding Flag-tagged Snrnp40

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Kvbeta K+ channel [K25/73R]

Plasmid

#128617

Purpose

Mammalian Expression Plasmid of anti-Pan-Kvbeta K+ channel. Derived from hybridoma K25/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Kvbeta K+ Channel Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-LC-Myosin-N-7

Plasmid

#54146

Purpose

Localization: Light Chain Myosin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

LC-Myosin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLENTI_TetOn_Hu_PTTG1(WT)

Plasmid

#136346

Purpose

Lentiviral inducible expression (TetOn) of mouse PTTG1

Depositor

Guillermo de Cárcer

Insert

PTTG1

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pE1B-C-F/Sox10-MCS8

Plasmid

#20247

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS8

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC2FG

Plasmid

#31281

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry2 (Cry2 Mouse)

Tags

Flag and His

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/nV5-DEST-Apr1

Plasmid

#20135

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pENTR-L1-CAMKII(0.4)-L4

Plasmid

#32579

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 0.4kb version

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Islr-AP-His

Plasmid

#71950

Purpose

Expresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr (Islr Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-3.6TPH-GAL4p65

Plasmid

#32571

Depositor

Sergey Kasparov

Article

Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

Insert

Gal4p65

Use

Lentiviral

Promoter

3.6TPH

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Promoter

PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-mCh-Chd1i4

Plasmid

#21908

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i4

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
XE93 mFZ3-CS2+

Plasmid

#16804

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ3-CS2+ (Fzd3 Mouse)

Use

Xenopus expression

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-mAIM2-PYD-EGFP

Plasmid

#51610

Purpose

Express GFP-tagged PYD of mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of HIN domain

Promoter

MESV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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