We narrowed to 14,039 results for: TIM

1. pYSD099

   Plasmid

   #212923

   Purpose

   A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. KG#901

   Plasmid

   #110936

   Purpose

   Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   mig-13 promoter
   Emerald-RAB-3
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2/NS4B

   Plasmid

   #120823

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS4B ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVT/Smarca4

   Plasmid

   #122146

   Purpose

   For making Smarca4 RNA

   Depositor

   Carmen Williams

   Article

   Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

   Insert

   Smarca4 (Smarca4 Mouse)
   

   Use

   For in vitro transcription

   Promoter

   T7

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2/NS3

   Plasmid

   #120822

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS3 ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPL5618_pUG_MtxR

   Plasmid

   #60929

   Purpose

   PCR template vector for generating methotrexate resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Dihydrofolate reductase (DHFR Human)
   

   Use

   Loxp flanked deletion cassette template

   Mutation

   L22F F31S

   Promoter

   TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFPV25.1_rpsM_mCerulean3

   Plasmid

   #124904

   Purpose

   rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

   Insert

   rpsM promoter and mCerulean3
   

   Expression

   Bacterial

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2/NS2B

   Plasmid

   #120819

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS2B ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

    Plasmid

    #220079

    Purpose

    SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    SACM1l-C389S-Fis1tail (SACM1L Human)
    

    Expression

    Mammalian

    Mutation

    C389SdeltaTMD

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-FOXO3a WT-ER

    Plasmid

    #8355

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    mouse estrogen receptor (G525R) on C-term

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pUCHR-inmCherry

    Plasmid

    #60239

    Purpose

    HIV-1 transfer vector that encodes mcherry reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inmCherry
    

    Use

    Lentiviral

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2/NS5-YFP

    Plasmid

    #120831

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP
    

    Tags

    eYFP-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV300

    Plasmid

    #204968

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ScFv-2ERT2-VPH

    Plasmid

    #120556

    Purpose

    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCMU-4

    Plasmid

    #172353

    Purpose

    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

    Depositor

    Eriko Takano

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    D4-TheoR-cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    D4

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

    Plasmid

    #197067

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    MitoTrap-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Promoter

    Fam208a

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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