We narrowed to 145,149 results for: ins

1. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

   Plasmid

   #185711

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed I770 to A

   Promoter

   T7

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Vav1-SH2

   Plasmid

   #46528

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Vav1 (VAV1 Human)
   

   Use

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 661-777

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBABE bleo mouse IRS-2

   Plasmid

   #11447

   Depositor

   Ronald Kahn

   Article

   Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

   Insert

   IRS-2 (Irs2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

4. B. fragilis mRNA toehold switch sensor

   Plasmid

   #110706

   Purpose

   Toehold switch sensor to detect a species specific mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. fragilis species specific toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C. difficile toxin B mRNA toehold switch sensor

   Plasmid

   #110715

   Purpose

   Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   C. difficile toxin B toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCarCBA

   Plasmid

   #117754

   Purpose

   Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac(UV5)

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-CmTMC1truncated

   Plasmid

   #154908

   Purpose

   For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-CmTMC1truncated
   

   Use

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-243 and 843-969

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-MuTMC2truncated

   Plasmid

   #154909

   Purpose

   For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-MuTMC2truncated
   

   Use

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-179 and 691-716

   Promoter

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. DYNLT1

   Plasmid

   #132528

   Purpose

   Human TCTEX-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLT1 (DYNLT1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid

    #127244

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX Eat2-SH2

    Plasmid

    #46423

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Eat2 (SH2D1B Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-132

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

    Plasmid

    #87202

    Purpose

    To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    UBL4A (UBL4A Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Residues from 90-107 are inserted.

    Promoter

    CMV

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX PLCg1(N)-SH2

    Plasmid

    #46470

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(N)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 539-664

    Promoter

    Tac

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

    Plasmid

    #138989

    Purpose

    IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-hygro-hPGAP3-3HA

    Plasmid

    #50373

    Purpose

    Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pACUH-GFP1-10 sec

    Plasmid

    #172062

    Purpose

    UAS vector to express GFP1-10 in the ER lumen

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP1-10 sec
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-mTAZ-WW (86)

    Plasmid

    #40915

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ 14-3-3 binding site and WW domain (…

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. C. difficile 16S toehold switch sensor

    Plasmid

    #110704

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

    Plasmid

    #131618

    Purpose

    Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM]
    NeoR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH028

    Plasmid

    #80393

    Purpose

    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    

    Use

    Synthetic Biology

    Tags

    Fused by GS linkers to 4 SH3 domains

    Expression

    Bacterial

    Mutation

    Glycine 448 changed to Alanine, makes it primaril…

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX Brk-SH2

    Plasmid

    #46411

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Brk (PTK6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 74-176

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-RlACR_477-EYFP

    Plasmid

    #103771

    Purpose

    Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_477
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Pr2-deGFP-MGapt

    Plasmid

    #67736

    Purpose

    OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr2

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Pr1-deGFP-MGapt

    Plasmid

    #67735

    Purpose

    OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr1

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX SHF-SH2

    Plasmid

    #46491

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHF (SHF Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 317-423

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX SHE-SH2

    Plasmid

    #46490

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHE
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 382-492

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX SHD-SH2

    Plasmid

    #46489

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHD (SHD Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 227-340

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Py2087ACR1

    Plasmid

    #153032

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    Py2087ACR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSTAP-seq_fly-zfh1

    Plasmid

    #86381

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX Rin3-SH2

    Plasmid

    #46476

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Rin3 (RIN3 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 55-157

    Promoter

    Tac

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge