We narrowed to 28,942 results for: Tat
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Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
Tags6HisExpressionMammalianMutationN516T, S533N and N539QAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-ccDockerin
Plasmid#72200PurposeMBP-ccDockerin expression plasmidDepositorInsertClostridium Cellulolyticum Type I Dockerin
TagsHis-Tag and Maltose Binding ProteinExpressionBacterialAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2711
Plasmid#175083PurposeMammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutationsDepositorInsertKSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationE223A, S226A, RC mutationsAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2712
Plasmid#175084PurposeMammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutationsDepositorInsertKSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationE223A, S226A, RN2 mutationsAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 Y635A
Plasmid#175772PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635ADepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 N637A
Plasmid#175773PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637ADepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
ExpressionBacterialMutationgfp with first two leucine codons mutated to amberAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585-gp130
Plasmid#174404PurposeBacterial expression of sarA-gp130 chimera under native promoter.DepositorInsertsarA-IL6ST chimera
ExpressionBacterialPromoterEndogenousAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBC1cer40GEM
Plasmid#172699PurposeUbiquitous expression of 60meric GEM monomerDepositorInsertmonomer of 60mer GEM
UseUnspecifiedTagsGFPExpressionPlantPromoterUBC1Available SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Nectin-2
Plasmid#170295PurposeA knock-out vector for the mouse Nectin-2DepositorInsertA gRNA targeting the mouse Nectin-2 gene.
UseCRISPR and LentiviralAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB-RM
Plasmid#168476PurposeN-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertN-fragment of DiB-RM
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI763 p(Z)-mCherry + UBC9 sgRNA
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRExpressionYeastPromoterP(Z), synthetic promoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPIC_pet28a
Plasmid#131654PurposeBacterial expression of ETS gene SPICDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N0
Plasmid#160983PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1-2A
Plasmid#160986PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2-2A
Plasmid#160987PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2
Plasmid#160985PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1
Plasmid#160984PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRExpressionBacterialPromoterPsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
Tags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2
Plasmid#91802PurposeExpresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY921
Plasmid#135092PurposeTranscriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
TagsGFP and Synthetic IntronExpressionWormPromoterPacr-2Available SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Asp73TAG
Plasmid#125266PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Asp73TAG
TagsHis-TagExpressionBacterialAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-nolinker-GFP
Plasmid#108579PurposeExpresses PKAcs-GFP fusion in mammalian cellsDepositorInsertprotein kinase A, catalytic subunit
TagsGFPExpressionMammalianMutationH87Q and W196RAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-G12X-GFP
Plasmid#108588PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
TagsGFPExpressionMammalianMutationH87Q and W196RAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 CaURA3 (pNTI592)
Plasmid#115431PurposeURA3-marked RPS28DepositorInsertsExpressionYeastMutationKpnI site in intronPromoterAshbya gossypii and endogenousAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
TagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArginineAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Gent
Plasmid#104522PurposeLI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.DepositorInsertApramycin resistance gene
UseCloning vectorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only