We narrowed to 9,415 results for: Coli

1. pACYCDuet-1:ChrA

   Plasmid

   #225079

   Purpose

   Expresses precursor peptide ChrA in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDae079 (eMutaT7[transition])

   Plasmid

   #187622

   Purpose

   Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Inserts

   TadA-8e-T7RNAP
   PmCDA1-T7RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEBB HA cIAP1

   Plasmid

   #38232

   Depositor

   Colin Duckett

   Article

   Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

   Insert

   cIAP1 (BIRC2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule-Tet3.0

   Plasmid

   #174080

   Purpose

   Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   Tet3.0 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p11d-sctRPA

   Plasmid

   #102614

   Purpose

   Expression of S. cerevisiea RPA in E.coli

   Depositor

   Marc Wold

   Article

   Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

   Inserts

   RPA1 (RFA1 Budding Yeast)
   RPA2 (RFA2 Budding Yeast)
   RPA3 (RFA3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   RFA2 has intron deleted

   Promoter

   T7

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSb_init containing the MBP sybody Sb_MBP#1

    Plasmid

    #132699

    Purpose

    expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    sybody_MBP#1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIntegrase_4

    Plasmid

    #60576

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST-2K

    Plasmid

    #44564

    Purpose

    for ectopic constitutive expression of two mycobacterial proteins in one vector

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNP1 sfGFP toehold switch

    Plasmid

    #107355

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold 7 + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAF

    Plasmid

    #104152

    Purpose

    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

    Depositor

    Robert Craigie

    Article

    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

    Insert

    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    

    Use

    Tags

    N-terminal His tag with thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DHFR-PP7-VP64_T2A_GFP

    Plasmid

    #86167

    Purpose

    Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-PP7-VP64_T2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized domain (N-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET151-hUCH37 (ISF1)

    Plasmid

    #61929

    Purpose

    Expression of hUCH37 (ISF1) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

23. Wild-type CoV-2 ORF8

    Plasmid

    #159933

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Use

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Use

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pCOLA banana sensor sfGFP

    Plasmid

    #107367

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    banana-detecting toehold + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ARK2aa

    Plasmid

    #81013

    Purpose

    E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-aphA
    and PMD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cr3Ec

    Plasmid

    #184860

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX3x-hRanBP2-(IR1+M)

    Plasmid

    #53140

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2631-2711)

    Promoter

    tac

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b/BOA

    Plasmid

    #82041

    Purpose

    For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

    Depositor

    Angela Gronenborn

    Article

    Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

    Insert

    Burkholderia oklahomensis agglutinin
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCas13 Twin-SUMO

    Plasmid

    #199753

    Purpose

    Expresses codon optimized miniature mCas13 for protein expression in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

    Insert

    mCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCOLA kiwi sensor sfGFP

    Plasmid

    #107368

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    kiwi-detecting toehold + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMJ915v2+Nterm Spe1 +Cterm Age1 site

    Plasmid

    #88914

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIntegrase_11

    Plasmid

    #60582

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-parkin 77-465

    Plasmid

    #45970

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 77-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pST-KNarK2

    Plasmid

    #44565

    Purpose

    for ectopic hypoxia inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBXPHM3-PCFT_NB

    Plasmid

    #165415

    Purpose

    Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT_NB (SLC46A1 Chicken, Llama)
    

    Use

    Tags

    His10-MBP-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    arabinose

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. His-Sin3a-115-212(pL01)

    Plasmid

    #124386

    Purpose

    E.Coli produced SIN3A PAH1 purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    His-Sin3a-115-212 (SIN3A )
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGEX-parkin W403A

    Plasmid

    #45974

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin W403A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEBB-HA-hILP del RING

    Plasmid

    #25675

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Encodes aa 1-449 only. aa 450-497 have been dele…

    Promoter

    Available Since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pGEX-parkin C431S

    Plasmid

    #45976

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin C431S (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET151-mUCH37 (ISF2)

    Plasmid

    #61928

    Purpose

    Expression of mUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    UCH37 (M.musculus) (Uchl5 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. V32M,L84M CoV-2 ORF8

    Plasmid

    #159934

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Use

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    V32M, L84M

    Promoter

    T7

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGEX-parkin F463A

    Plasmid

    #45975

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin F463A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRSET-GST-05230

    Plasmid

    #67874

    Purpose

    E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

    Depositor

    Armin Djamei

    Article

    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

    Insert

    shy1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pNP1 OR sfGFP

    Plasmid

    #107364

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    OR gate + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pNL1 Ecarin

    Plasmid

    #106289

    Purpose

    Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Ecarin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge