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Showing: 8801 - 8850 of 13580 results

  1. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  2. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  3. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211343
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211343-plasmid-map-sequence-id-415647

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  4. GBH-2aG4-gcryBFP.1

    Plasmid
    #188013
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188013-plasmid-map-sequence-id-381108

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  5. GBH-2aG4-gcryBFP.2

    Plasmid
    #188014
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188014-plasmid-map-sequence-id-381045

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  6. GBH-2aG4-gcryGFP.1

    Plasmid
    #188016
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188016-plasmid-map-sequence-id-381139

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  7. GBH-2aG4-gcryGFP.2

    Plasmid
    #188017
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188017-plasmid-map-sequence-id-381140

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  8. GBH-2aG4-gcryGFP.3

    Plasmid
    #188018
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188018-plasmid-map-sequence-id-381141

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  9. pT7CFE1_EVD68_VP12A_C107R

    Plasmid
    #212982
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A C107R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 107 from 2A to Arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212982-plasmid-map-sequence-id-422577

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  10. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

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  11. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

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  12. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

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  13. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid
    #203710
    Purpose
    Overexpression of FLAG-S1PR1-S5A mutant.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-S5A (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S5A
    Promoter
    CMV
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203710-plasmid-map-sequence-id-403867

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  14. pET3-ARF6-His6(CO)

    Plasmid
    #201887
    Purpose
    Used for bacterial expression of human protein ARF6
    Depositor
    Richard Kahn
    Insert
    ARF6 (ARF6 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201887-plasmid-map-sequence-id-403824

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  15. pET3-ARL1-His6 (CO)

    Plasmid
    #201866
    Purpose
    Used for bacterial expression of human protein ARL1
    Depositor
    Richard Kahn
    Insert
    ARL1 (ARL1 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201866-plasmid-map-sequence-id-403817

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  16. pET3-[Δ1-32]ARL10-His6(CO)

    Plasmid
    #201876
    Purpose
    Used for bacterial expression of human protein ARL10
    Depositor
    Richard Kahn
    Insert
    ARL10 (ARL10 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Deletion of residues 1-32 from N-terminus and cod…
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201876-plasmid-map-sequence-id-403811

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  17. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid
    #197377
    Purpose
    The AAV vector for PhyB621(Y276H) and m1V
    Depositor
    Michiyuki Matsuda
    Insert
    Phytochrome B Y276H(1-621 aa)
    Use
    AAV
    Tags
    P2A-m1V
    Expression
    Mutation
    Human codon optimized PhyB (1-621 aa), Y276H muta…
    Promoter
    CMV
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197377-plasmid-map-sequence-id-389799

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  18. pET3-ARF4-His6(CO)

    Plasmid
    #201885
    Purpose
    Used for bacterial expression of human protein ARF4
    Depositor
    Richard Kahn
    Insert
    ARF4 (ARF4 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201885-plasmid-map-sequence-id-403153

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  19. pET3-ARF3-His6(CO)

    Plasmid
    #201884
    Purpose
    Used for bacterial expression of human protein ARF3
    Depositor
    Richard Kahn
    Insert
    ARF3 (ARF3 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201884-plasmid-map-sequence-id-402538

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  20. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

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  21. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  22. pHH0103 CEP164 WW domain

    Plasmid
    #104276
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104276-plasmid-map-sequence-id-203766

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  23. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104300-plasmid-map-sequence-id-203483

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  24. pHH0103 HECW2 WW domain #1

    Plasmid
    #104244
    Purpose
    Bacterial expression of WW domain #1 from HECW2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HECW2 WW domain #1 (HECW2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104244-plasmid-map-sequence-id-204378

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  31. pCMV-T7-ABEmax-SpRY

    Plasmid
    #195279
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
    Depositor
    Petros Patsali, Carsten Lederer
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195279-plasmid-map-sequence-id-394869

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  32. pCDH-Flag-hS1pr1-GFP-Neo

    Plasmid
    #203708
    Purpose
    Overexpression of FLAG-S1PR1-GFP fusion.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-GFP (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag and GFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Aug. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203708-plasmid-map-sequence-id-403683

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  36. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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  37. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

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  40. TAF1L

    Plasmid
    #39014
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1L (TAF1L Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200420-plasmid-map-sequence-id-403933

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  48. pIA034

    Plasmid
    #200421
    Purpose
    amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200421-plasmid-map-sequence-id-405458

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  49. R777-E112 Hs.IRS2-nostop

    Plasmid
    #70396
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70396-plasmid-map-sequence-id-125845

    Enlarge

Showing: 8801 - 8850 of 13580 results