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We narrowed to 14,145 results for: TIM

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Showing: 8801 - 8850 of 14145 results

  5. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  7. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  8. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  9. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available Since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172908-plasmid-map-sequence-id-341477

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  14. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  15. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  16. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  20. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  21. pGENT_GW_aco_mScarlet3

    Plasmid
    #225991
    Purpose
    To make gene fusions with Arabidopsis codon-optimized mScarlet3
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Tags
    mScarlet3
    Expression
    Plant
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225991-plasmid-map-sequence-id-448012

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  22. FgH1tUTCherry

    Plasmid
    #85552
    Purpose
    Inducible expression of guide with fluorescent Cherry reporter
    Depositor
    Marco Herold
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85552-plasmid-map-sequence-id-169902

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  24. FgH1tUTCyan

    Plasmid
    #85551
    Purpose
    Inducible expression of guide with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    H1t
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85551-plasmid-map-sequence-id-169908

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  30. pLXIN2

    Plasmid
    #99203
    Purpose
    Bicistronic retroviral vector (optimized internal ribosome entry site)
    Depositor
    Saroj Mathupala
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    LTR
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99203-plasmid-map-sequence-id-192445

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  31. FRP935

    Plasmid
    #159302
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159302-plasmid-map-sequence-id-311152

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  32. FRP1999

    Plasmid
    #159308
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    159308-plasmid-map-sequence-id-311207

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  33. FRP2000

    Plasmid
    #159309
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159309-plasmid-map-sequence-id-311209

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  34. FRP1975

    Plasmid
    #159310
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mNeongreen under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159310-plasmid-map-sequence-id-311210

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  35. FRP2002

    Plasmid
    #159311
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159311-plasmid-map-sequence-id-311211

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  36. FRP266

    Plasmid
    #159304
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mKate2 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159304-plasmid-map-sequence-id-311199

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  37. FRP265

    Plasmid
    #159301
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mTFP1 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159301-plasmid-map-sequence-id-311095

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  38. FRP1931

    Plasmid
    #159306
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mRuby2 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159306-plasmid-map-sequence-id-311203

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  39. pORFE0001

    Plasmid
    #112070
    Purpose
    AtCas9 module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112070-plasmid-map-sequence-id-217624

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  40. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

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  41. FRP2008

    Plasmid
    #159312
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    V2del
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159312-plasmid-map-sequence-id-311212

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  42. FRP2010

    Plasmid
    #159313
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Mutation
    V2del, L231H
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159313-plasmid-map-sequence-id-311213

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  43. pPB-CAG-rtTA3G-IH

    Plasmid
    #214118
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    trTA3G
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214118-plasmid-map-sequence-id-423610

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  44. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    hUBC
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85555-plasmid-map-sequence-id-169910

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  45. pPB-CAG-rtTAM2-IB

    Plasmid
    #214117
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    rtTAM2
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214117-plasmid-map-sequence-id-423608

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  46. FUCas9Cyan

    Plasmid
    #85554
    Purpose
    Expresses Cas9 with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyrogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    hUBC
    Available Since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85554-plasmid-map-sequence-id-180892

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  47. FRP1864

    Plasmid
    #159307
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159307-plasmid-map-sequence-id-311204

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  48. pORFE1001

    Plasmid
    #112079
    Purpose
    Overexpression of the AtCAS9 in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112079-plasmid-map-sequence-id-217610

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  49. CSII-CAG-EGFP

    Plasmid
    #214112
    Purpose
    Mammalian continuous expression
    Depositor
    Sho Yoshimatsu
    Insert
    EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214112-plasmid-map-sequence-id-423582

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  50. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Tags
    None
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

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Showing: 8801 - 8850 of 14145 results