We narrowed to 8,264 results for: 11
-
Plasmid#122261PurposepBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015DepositorInsertpBRBAD, pBRlasI
UseSynthetic BiologyAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS1479-HTP-KanMX6
Plasmid#154085PurposeFor C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.DepositorInserthis6-TEV-protein A-KanR
UsePcr-based tagging in yeastTagshis6-TEV-protein AAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT
Plasmid#140205PurposeProof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmCherry
sgRNA E1
UseCRISPR and Synthetic Biology; Proof-of-concept te…MutationG174DPromoterPelcA and U3Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
TR- ATG-HP-GFP
Plasmid#80592PurposeExpresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI165
Plasmid#126074PurposeVector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsYFP1-10 (improved)ExpressionPlantAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJNKsub(TA)DD-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-ClpX
Plasmid#89657PurposePlasmid for titrating synthesis of ClpX with doxycyclineDepositorInsertclpX
UseSynthetic BiologyAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPKsub(TA)-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Evf2
Plasmid#99478PurposeMammalian expression of the Evf2 long non-coding RNADepositorInsertEvf2
ExpressionMammalianAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-466
Plasmid#92251PurposePlasmid for expression of GST-tagged human TNRC6C CED in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
TagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-3
Plasmid#89697Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-4
Plasmid#89698Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFosill-2
Plasmid#89696Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pconst #2-RFP
Plasmid#81076Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter- BBa_J23104Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cx26-sfGFP
Plasmid#69026PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCx26 (Gjb2 Rat)
Tagsnon-monomerized superfolder GFP (V206)ExpressionMammalianPromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU2-Pleum
Plasmid#60328PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TALEN-CCR5L18-CtermCan
Plasmid#51438PurposeExpresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Can C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermCan
Plasmid#51439PurposeExpresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Can C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only